Application of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Discrimination of Escherichia Strains Possessing Highly Conserved Ribosomal RNA Gene Sequences
スポンサーリンク
概要
- 論文の詳細を見る
We evaluated the capability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to discriminate twelve Escherichia strains: E. blattae, E. fergusonii, E. hermanii and nine E. coli, whose ribosomal RNA (rRNA) gene sequence homologies are in the range of 96—100%. Similarities obtained by MALDI-TOF MS were found to be 78—92% among the E. coli strains, and 74% between E. coli and E. fergusonii. E. blattae and E. hermanii showed only 32% similarity when compared to the other species. Thus, MALDI-TOF MS provides capability of distinguishing bacterial species or even strains possessing highly conserved rRNA gene sequences.
- 日本薬学会の論文
著者
-
Muroi Masashi
Research Institute of Pharmaceutical Sciences, Musashino University
-
Shima Keisuke
Analytical & Measuring Instruments Division, Shimadzu Corporation
-
Nakagawa Yasuyoshi
Resource Collection Division, National Institute of Technology and Evaluation
-
Tanamoto Ken-ichi
Research Institute of Pharmaceutical Sciences, Musashino University
-
Tanamoto Ken-ichi
Research Institute Of Pharmaceutical Sciences Musashino University
-
Muroi Masashi
Research Institute Of Pharmaceutical Sciences Musashino University
関連論文
- Urban Aerosols Induce Pro-inflammatory Cytokine Production in Macrophages and Cause Airway Inflammation in Vivo
- Application of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Discrimination of Escherichia Strains Possessing Highly Conserved Ribosomal RNA Gene Sequences
- Inhibitory effects of soluble MD-2 and soluble CD14 on bacterial growth
- Application of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Discrimination of Laboratory-Derived Antibiotic-Resistant Bacteria
- Toll-Like Receptors Required for Dermatophagoides farinae to Activate NF-κB