Proliferation of Canine Intervertebral Disk Chondrocytes in Three-Dimensional Alginate Microsphere Culture.

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概要

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• Proliferation of chondrocytes from nucleus pulposus (NP) and anulus fibrosus (AF) was confirmed in three-dimensional culture using alginate microspheres. Cells isolated from NP and AF were incorporated in microspheres and cultured for 14 days. Round mononuclear cells of 20-25 μm in diameter proliferated and formed aggregates. At day 14, alcian blue positive matrix surrounded the proliferating cells. The cells had cytoplasmic vacuoles stained positively by toluidine blue. On electron microscopy, the cells contained proteoglycan vacuoles and lipid droplets in the cytoplasm and synthesized collagen fibrils and electron dense granules surrounding the cell. These features of the cells were characteristic for chondrocytes. This culture system should be useful to further investigate metabolic activities of intervertebral disk chondrocytes.

• 公益社団法人 日本獣医学会の論文

著者

• Masuda Kenichi

  Department Of Orthopaedic Surgery Kansai College Of Oriental Medicine

• MEACHUM Sharon

  Department of Veterinary Biosciences, College of Veterinary Medicine, University of Illinois

• MIYABAYASHI Takayoshi

  Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois

• EURELL Thomas

  Department of Veterinary Biosciences, College of Veterinary Medicine, University of Illinois

• MASUDA Kenichi

  Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois

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