Chicken B Lymphoma DT40 Cells as a Useful Tool for in vitro Analysis of Pathogenic Infectious Bursal Disease Virus
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概要
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Susceptibility of DT40 cells to pathogenic field strains of infectious bursal disease virus (IBDV) including very virulent and classical virulent strains were studied. After the first and second passage of the virus in DT40 cells, IBDV-specific antigen was readily detected in DT40 cells inoculated with the pathogenic field strain infected bursal homogenates. Nucleotide sequence analysis in the VP2 hypervariable domain, which is critical for the virulence of IBDV, revealed no common amino acid substitutions among the pathogenic IBDVs in accordance with the propagation in DT40 cells. These results indicate that DT40 cells are a useful tool for rapid isolation of pathogenic field strains and successive in vitro analysis of IBDV.
著者
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FUKUSHI Hideto
Department of Applied Veterinary Science, United Graduate School of Veterinary Sciences
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Ohya Kenji
Laboratory Of Infectious Diseases Department Of Disease Control Graduate School Of Veterinary Medici
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Yamaguchi Tsuyoshi
The Avian Zoonosis Research Center Faculty Of Agriculture Tottori University
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Hirayama Haruko
Laboratory Of Physiology Department Of Basic Veterinary Science The United Graduate School Of Veteri
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KASANGA Christopher
Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University
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MAW Min
Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University
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TERASAKI Kaori
Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University
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HIRAYAMA Haruko
Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University
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