Single channel properties of lysenin measured in artificial lipid bilayers and their applications to biomolecule detection
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概要
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Single channel currents of lysenin were measured using artificial lipid bilayers formed on a glass micropipette tip. The single channel conductance for KCl, NaCl, CaCl2, and Trimethylammonium-Cl were 474 ± 87, 537 ± 66, 210 ± 14, and 274 ± 10 pS, respectively, while the permeability ratio PNa/PCl was 5.8. By adding poly(deoxy adenine) or poly(L-lysine) to one side of the bilayer, channel currents were influenced when membrane voltages were applied to pass the charged molecules through the channel pores. Current inhibition process was concentration-dependent with applied DNA. As the current fluctuations of α-hemolysin channels is often cited as the detector in a molecular sensor, these results suggest that by monitoring channel current changes, the lysenin channel has possibilities to detect interactions between it and certain biomolecules by its current fluctuations.(Communicated by Fumio OOSAWA, M.J.A.)
著者
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Aoki Takaaki
Network Center For Molecular And System Life Sciences Graduate School Of Frontier Bioscience Osaka U
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Kobayashi Toshihide
Lipid Biology Lab., RIKEN
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Kobayashi Toshihide
Lipid Biology Lab. Riken
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Hirano Minako
Graduate School Of Frontier Biosciences Osaka University:riken Advanced Science Institute
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Takeuchi Yuko
Graduate School Of Frontier Biosciences Osaka University
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HIRANO Minako
Laboratory for Nanobiology, Graduate School of Frontier Biosciences, Osaka University
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TAKEUCHI Yuko
Network Center for Molecular and System Life Science, Graduate School of Frontier Biosciences, Osaka
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YANAGIDA Toshio
Network Center for Molecular and System Life Science, Graduate School of Frontier Biosciences, Osaka
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IDE Toru
Network Center for Molecular and System Life Science, Graduate School of Frontier Biosciences, Osaka
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AOKI Takaaki
Network Center for Molecular and System Life Science, Graduate School of Frontier Biosciences, Osaka University
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YANAGIDA Toshio
Network Center for Molecular and System Life Science, Graduate School of Frontier Biosciences, Osaka University
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Kobayashi Toshihide
Lipid Bio. Lab., RIKEN
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KOBAYASHI Toshihide
Lipid Biology Laboratory, RIKEN
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TAKEUCHI Yuko
Network Center for Molecular and System Life Science, Graduate School of Frontier Biosciences, Osaka University
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IDE Toru
Network Center for Molecular and System Life Science, Graduate School of Frontier Biosciences, Osaka University
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