Selective Analysis of Lipids by Thin-Layer Chromatography Blot Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry
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概要
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Thin-layer chromatography (TLC) is an essential method for food composition analyses such as lipid nutrition analysis. TLC can be used to obtain information about the lipid composition of foods; however, it cannot be used for analyses at the molecular level. Recently we developed a new method that combines matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) with TLC-blotting (TLC-Blot-MALDI-IMS). The combination of MALDI-IMS and TLC blotting enabled detailed and sensitive analyses of lipids. In this study, we applied TLC-Blot-MALDI-IMS for analysis of major phospholipids extracted from bluefin tuna. We showed that TLC-Blot-MALDI-IMS analysis could visualize and identify major phospholipids such as phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylcholine, and sphingomyelin.
著者
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Setou Mitsutoshi
Department of Molecular Anatomy, Hamamatsu University School of Medicine
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Setou Mitsutoshi
Department Of Bioscience And Biotechnology Tokyo Institute Of Technology
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ZAIMA Nobuhiro
Department of Molecular Anatomy, Hamamatsu University School of Medicine
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ZAIMA Nobuhiro
Mitsubishi Kagaku Institute of Life Sciences (MITILS)
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Goto-Inoue Naoko
Department of Molecular Anatomy, Hamamatsu University School of Medicine
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Adachi Kohsuke
Graduate School of Agriculture, Kochi University
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Goto‐inoue Naoko
Hamamatsu Univ. School Of Medicine Shizuoka Jpn
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Zaima Nobuturo
Department Of Molecular Anatomy Hamamatsu University School Of Medicine
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Zaima Nobuhiro
Department Of Applied Biological Chemistry Graduate School Of Agriculture Kinki University
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Goto-Inoue Naoko
Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University
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