A CMP-N-acetylneuraminic Acid Synthetase Purified from a Marine Bacterium, Photobacterium leiognathi JT-SHIZ-145
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概要
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A cytidine 5′-monophospho-N-acetylneuraminic acid (CMP-Neu5Ac) synthetase was found in a crude extract prepared from Photobacterium leiognathi JT-SHIZ-145, a marine bacterium that also produces a β-galactoside α2,6-sialyltransferase. The CMP-Neu5Ac synthetase was purified from the crude extract of the cells by a combination of anion-exchange and gel filtration column chromatography. The purified enzyme migrated as a single band (60 kDa) on sodium dodecylsulfate–polyacrylamide gel electrophoresis. The activity of the enzyme was maximal at 35 °C at pH 9.0, and the synthetase required Mg2+ for activity. Although these properties are similar to those of other CMP-Neu5Ac synthetases isolated from bacteria, this synthetase produced not only CMP-Neu5Ac from cytidine triphosphate and Neu5Ac, but also CMP-N-glycolylneuraminic acid from cytidine triphosphate and N-glycolylneuraminic acid, unlike CMP-Neu5Ac synthetase purified from Escherichia coli.
著者
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Kajiwara Hitomi
Glycotechnology Business Unit, Japan Tobacco Inc.
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Mine Toshiki
Glycotechnology Business Unit, Japan Tobacco Inc.
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Yamamoto Takeshi
Glycotechnology Business Unit, Japan Tobacco Inc.
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MIYAZAKI Tatsuo
Department of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences
関連論文
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- A CMP-N-acetylneuraminic acid synthetase purified from a marine bacterium, Photobacterium leiognathi JT-SHIZ-145
- A CMP-N-acetylneuraminic Acid Synthetase Purified from a Marine Bacterium, Photobacterium leiognathi JT-SHIZ-145
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- A CMP-N-acetylneuraminic Acid Synthetase Purified from a Marine Bacterium, Photobacterium leiognathi JT-SHIZ-145
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