Reduction of misacylation to the amber suppressor tRNA during site-specific incorporation of unnatural amino acids

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概要

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• Incorporations of unnatural amino acids in proteins are a useful technique in medical and industrial fields. For practical usages, misacylation caused by engineered aminoacyl-tRNA synthetases can be one of the issues lowering the purity of the generated proteins. Since acceptor stems of tRNAs are known to possess identity elements specific for each amino acid, the misacylation is probably caused by non-specificity of the acceptor stems in tRNAs. In this paper, we modified an acceptor stem of tRNATyr, and tested aminoacylation of over 100 modified tRNAs. Finally, we found the acceptor stem sequence for the tRNA with less misacylation for both tyrosine and tyrosine derivative unnatural amino acids.

著者

• FUJII Akihiko

  Institute of Industrial Science, The University of Tokyo

• ONODERA Kenji

  Institute of Industrial Science, The University of Tokyo

• KAMIJO Shunsuke

  Institute of Industrial Science, The University of Tokyo

• Fujii Akihiko

  Institute For Nuclear Study University Of Tokyo : Department Of Physics School Of Science And Techno

• Onodera Kenji

  The University Of Tokyo Institute Of Industrial Science

• Kamijo Shunsuke

  Institute Of Industrial Science The University Of Tokyo

• Onodera Kenji

  Institute Of Industrial Science The University Of Tokyo

関連論文

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