Medicinal Herb Research: Serum Pharmacological Method and Plasma Pharmacological Method

スポンサーリンク

概要

• 論文の詳細を見る

• Serum pharmacological method has generally been used in herb studies. However, preparation of test serum for ex vivo experiment is an intricate process: besides pretreatment (heat or chemicals), it involves the proteolytic cascades of coagulation along with fibrinolysis, complement and kinin systems, as well as platelet and leukocyte activation resulting in release reactions. These processes deviate serum sample components away from the original in vivo state, and possibly also have effects on the absorbed herbal components and their downstream effectors in blood. The conclusions drawn from serum pharmacological method are at least partially uncertain in its validity. These processes can be avoided by anticoagulation. Compared to those of the serum, constituents of plasma are better reflectors of the in vivo physiological/pathological state and medicinal herb-induced changes. Therefore, we have advocated the adoption of plasma pharmacological method in ex vivo experiments of herb studies. Recent studies including our work demonstrated that the constituents and biological activities are partially different between absorbed medicinal herbs in plasma and serum. This review summarizes the experimental evidence supporting the feasibility of plasma pharmacological method and discusses the reasons and facts that flaw the serum pharmacological method. But serum pharmacological method can be used if anticoagulants interfere with experiments. It should be emphasized that the domination between plasma and serum pharmacological methods is different depending on the usage. Indeed, the pros and cons of both methods as well as the appropriate choices of coagulants in different ex vivo experimental settings remain to be further elucidated.

著者

• Wang Yuhong

  Department Of Anatomy Biology And Medicine Oita University School Of Medicine

• He Rong

  Department Of Electronics And Mechanical Engineering Chiba University

• Ge Jinwen

  Department of Vascular Biology, Hunan University of Chinese Medicine

• Wang Dongsheng

  Department of Integrative Medicine, Xiangya Hospital / Central South University

• Zhu Huibin

  Department of Vascular Biology, Hunan University of Chinese Medicine

• He Shilin

  Hemostasis Physiology Lab., Xiangya Medical School / Central South University

• Wang Yuhong

  Department of Vascular Biology, Hunan University of Chinese Medicine

• WANG Dongsheng

  Department of Computer Science and Technology, Tsinghua University, and Tsinghua National Laboratory for Information Science and Technology

関連論文

• Medicinal Herb Research: Serum Pharmacological Method and Plasma Pharmacological Method
• A Nonlinear Output Feedback Control Method for a 5DOF Active Magnetic Bearing System
• SYNTHESIS OF ω-TERT-BUTYL ESTERS OF ASPARTIC ACID AND GLUTAMIC ACID VIA B, B-DIFLUOROBOROXAZOLIDONES
• Characterization and Expression of Serotonin Transporter Genes in Zebrafish
• LPV Modelling and Gain-Scheduled Control Approach for the Transient Stabilization of Power Systems
• Bayesian Theory Based Adaptive Proximity Data Accessing for CMP Caches

スポンサーリンク