Enhanced osteoblastic adhesion through improved wettability on polarized hydroxyapatite

スポンサーリンク

概要

• 論文の詳細を見る

• Osteoblasts are susceptible to the surface characteristics of bioceramics and stimulation from outside the cells. The purpose of this study was to evaluate the effects of electrical polarization on surface characteristics and osteoblastic adhesion. The surface characteristics revealed that electrical polarization had no effect on surface roughness, crystallinity, and constituent elements. According to contact angle measurement, electrically polarized HA, which provides two kinds of surfaces, negatively charged HA (N-HA) and positively charged HA (P-HA), was even more hydrophilic than that of normal HA (O-HA). Morphological observations and quantitative analyses revealed that the typical adhered cells had a round shape on the O-HA but had a spindle spreading configuration on the N-HA and the P-HA 1 h after seeding. After 3 h of cultivation, the rate of the number of spread cells on the O-HA increased and approached that of the N-HA and P-HA. However, the cell areas positively stained for actin, which indicates the degree of cell spreading, were distinctly larger on the N-HA and P-HA than that on the O-HA. The number of focal adhesions per cell was also less than that on the N-HA and P-HA. To conclude, the polarization improved the wettability of the HA surface, which consequently affected osteoblastic adhesion, especially spreading.

• 公益社団法人 日本セラミックス協会の論文

著者

• NAGAI Akiko

  Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University

• YAMASHITA Kimihiro

  Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University

• NAKAMURA Miho

  Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University

• Nagai Akiko

  Institute Of Biomaterials And Bioengineering Department Of Inorganic Materials Tokyo Medical And Den

• OKURA Toshinori

  Environmental and Energy Chemistry, Kogakuin University

• SEKIJIMA Yasutaka

  Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University

• HENTUNEN Teuvo

  Institute of Biomedicine, University of Turku

• Yamashita Kimihiro

  Institute Of Biomaterials And Bioengineering Department Of Inorganic Materials Tokyo Medical And Den

関連論文

• Self-sealing ability of OCP-mediated cement as a deciduous root canal filling material
• In vitro short-term platelet adhesion on various metals
• 11-O-14 Cell Activation on Biocomposite Scaffold Formed on Biomaterials
• Conduction properties of non-stoichiometric hydroxyapatite whiskers for biomedical use
• Electrovector effect on bone-like apatite crystal growth on Inside pores of polarized porous hydroxyapatite ceramics in simulated body fluid( Ceramics for Biomedical Applications in Asian Countries)
• Enhanced Bioactivity of 45S5 Type Glass by Electrical Polarization Treatment
• Enhanced osteoblastic adhesion through improved wettability on polarized hydroxyapatite
• Machinable glass-ceramics forming as a restorative dental material
• Modulation of Osteoblast-Like Cell Behavior Cultured on Hydroxyapatite by Thrombin
• Role of Collagen and Inorganic Components in Electrical Polarizability of Bone

スポンサーリンク