Modulation of Osteoblast-Like Cell Behavior Cultured on Hydroxyapatite by Thrombin
スポンサーリンク
概要
- 論文の詳細を見る
Blood proteins have been shown to be effective for osteoconduction and for biomaterial applications. The blood coagulation system progressed rapidly after biomaterial implantation. The interaction of biomaterials with blood coagulation proteins is considered to play an important role in osteoconduction. The purpose of this study was to evaluate the in vitro interaction of the coagulation factor with hydroxyapatite (HA) and the interaction of the coagulation factor with osteoblast-like cells cultured on HA. After 0.2 U/ml thrombin stimulation, the proliferation, alkaline phosphatase (ALP) activity, adherent morphology, and tyrosine phosphorylation of the osteoblast-like cells cultured on HA samples were investigated. Incubation with 0.2 U/ml thrombin stimulation induced an increase in cell number and inhibition of ALP activity. The thrombin stimulation resulted in changes in the morphology of the adherent osteoblast-like cells and static bands recognized as tyrosine phosphorylated proteins lysed from adherent osteoblast-like cells in blot analysis. These results of this study indicated that the thrombin promoted the proliferation, changes of cell morphology, and inhibition of ALP activity. It was suggested that thrombin could affect the cell morphology and proliferation near the HA.
著者
-
Tanaka Yumi
Institute Of Biomaterials And Bioengineering Department Of Inorganic Materials Tokyo Medical And Den
-
NAGAI Akiko
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
-
NAKAMURA Miho
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
-
Nagai Akiko
Institute Of Biomaterials And Bioengineering Department Of Inorganic Materials Tokyo Medical And Den
-
SEKIJIMA Yasutaka
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
-
Yamashita Kimihiro
Institute Of Biomaterials And Bioengineering Department Of Inorganic Materials Tokyo Medical And Den
-
Nakamura Satoshi
Institute for Medical and Dental Engineering, Tokyo Medical and Dental University
-
TANAKA Yumi
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
-
OHASHI Natalie
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
関連論文
- Self-sealing ability of OCP-mediated cement as a deciduous root canal filling material
- In vitro short-term platelet adhesion on various metals
- Biocompatibility and water durability of alumina-zirconia ceramics blended with microsized HA particles
- Conduction properties of non-stoichiometric hydroxyapatite whiskers for biomedical use
- Electrovector effect on bone-like apatite crystal growth on Inside pores of polarized porous hydroxyapatite ceramics in simulated body fluid( Ceramics for Biomedical Applications in Asian Countries)
- Phase Transition of Monoclinic Hydroxyapatite
- Enhanced Bioactivity of 45S5 Type Glass by Electrical Polarization Treatment
- Enhanced osteoblastic adhesion through improved wettability on polarized hydroxyapatite
- Flashing Flow of LiBr Aqueous Solution in a Convergent-Divergent Nozzle : Flow characteristics in nozzle and photographic observations of droplet jet from nozzle exit
- Machinable glass-ceramics forming as a restorative dental material
- Modulation of Osteoblast-Like Cell Behavior Cultured on Hydroxyapatite by Thrombin
- Superconductivity due to Two Dimensional Antiferromagnetic Spin Fluctuation including Vertex Corrections : Condensed Matter: Electronic Properties, etc.
- Role of Collagen and Inorganic Components in Electrical Polarizability of Bone