Identification of Salt-Tolerant Sinorhizobium sp. Strain BL3 Membrane Proteins Based on Proteomics

スポンサーリンク

概要

• 論文の詳細を見る

• Sinorhizobium sp. BL3 is a salt-tolerant strain that can fix atmospheric nitrogen in symbiosis with leguminous host plants under salt-stress conditions. Since cell membranes are the first barrier to environmental change, it is interesting to explore the membrane proteins within this protective barrier under salt stress. The protein contents of membrane-enriched fractions obtained from BL3 were analyzed by nanoflow liquid chromatography interfaced with electrospray ionization tandem mass spectrometry. A total of 105 membrane proteins were identified. These proteins could be classified into 17 functional categories, the two biggest of which were energy production and conversion, and proteins not in clusters of orthologous groups (COGs). In addition, a comparative analysis of membrane proteins between salt-stressed and non-stressed BL3 cells was conducted using a membrane enrichment method and off-line SCX fractionation coupled to nanoLC-MS/MS. These techniques would be useful for further comparative analysis of membrane proteins that function in the response to environmental stress.

• 日本微生物生態学会・日本土壌微生物学会の論文

著者

• Teaumroong Neung

  School Of Biotechnology Institute Of Agricultural Technology Suranaree University Of Technology

• Boonkerd Nantakorn

  School Of Biotechnology Institute Of Agricultural Technology Suranaree University Of Technology

• Jensen Ole

  Department Of Biochemistry And Molecular Biology University Of Southern Denmark

• TANTHANUCH WARAPORN

  Synchrotron Light Research Institute (Public Organization)

• TITTABUTR PANLADA

  School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology

• Matthiesen Rune

  Bioinformatics Unit-cic Biogune Parque Tecnologico De Bizkaia Edificio

• Mohammed Shabaz

  Biomolecular Mass Spectrometry And Proteomics Group Bijvoet Center For Biomolecular Research And Utr

• Manassila Monchai

  School Of Biotechnology Institute Of Agricultural Technology Suranaree University Of Technology

• Yamabhai Montarop

  School Of Biotechnology Institute Of Agricultural Technology Suranaree University Of Technology

• Tittabutr Panlada

  School Of Biotechnology Institute Of Agricultural Technology Suranaree University Of Technology

• Matthiesen Rune

  Bioinformatics Unit—CIC bioGUNE, Parque Tecnológico de Bizkaia Edificio

関連論文

• Changes in Bacterial Community Composition in the System of Rice Intensification (SRI) in Chiang Mai, Thailand
• Identification of Salt-Tolerant Sinorhizobium sp. Strain BL3 Membrane Proteins Based on Proteomics
• Rates of Lumbar Disc Surgery Before and After Implementation of Multidisciplinary Nonsurgical Spine Clinics
• Identification of Salt-Tolerant Sinorhizobium sp. Strain BL3 Membrane Proteins Based on Proteomics
• One-Year Follow-Up in Employees Sick-Listed Because of Low Back Pain : Randomized Clinical Trial Comparing Multidisciplinary and Brief Intervention
• Effect of Rice Cultivation Systems on Indigenous Arbuscular Mycorrhizal Fungal Community Structure
• Alleviation of the effect of environmental stresses using co-inoculation of mungbean by Bradyrhizobium and rhizobacteria containing stress-induced ACC deaminase enzyme(Soil biology)

スポンサーリンク