A Simple in Situ Cell-Based SUMOylation Assay with Potential Application to Drug Screening

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概要

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• We found that the small ubiquitin-related modifier (SUMO) conjugation reaction can be visualized simply by incubating green fluorescent protein (GFP)-SUMO-1 with permeabilized cells in the presence of ATP for 15 min. Neither special equipment for protein purification nor highly developed skills for recombinant technologies is required, making the assay potentially applicable to large-scale drug-screening strategies for the identification of drugs that can inhibit or enhance SUMOylation.

著者

• Muramatsu Miyuki

  Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University

• Uwada Junsuke

  Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University

• Matsumoto Naoyuki

  Faculty of Science, Kumamoto University

• SAITOH Hisato

  Global COE (Centers of Excellence) Program, Global Initiative Center for Pulsed Power Engineering, K

関連論文

• A Simple in Situ Cell-Based SUMOylation Assay with Potential Application to Drug Screening
• A Simple in Situ Cell-Based SUMOylation Assay with Potential Application to Drug Screening

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