A Simple in Situ Cell-Based SUMOylation Assay with Potential Application to Drug Screening
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概要
- 論文の詳細を見る
We found that the small ubiquitin-related modifier (SUMO) conjugation reaction can be visualized simply by incubating green fluorescent protein (GFP)-SUMO-1 with permeabilized cells in the presence of ATP for 15 min. Neither special equipment for protein purification nor highly developed skills for recombinant technologies is required, making the assay potentially applicable to large-scale drug-screening strategies for the identification of drugs that can inhibit or enhance SUMOylation.
- 2010-07-23
著者
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Uwada Junsuke
Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University
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Matsumoto Naoyuki
Faculty of Science, Kumamoto University
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Uwada Junsuke
Department Of Biological Sciences Graduate School Of Science And Technology Kumamoto University
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Saitoh Hisato
Global Coe (centers Of Excellence) Program Global Initiative Center For Pulsed Power Engineering Kum
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Muramatsu Miyuki
Department Of Biological Sciences Graduate School Of Science And Technology Kumamoto University
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Matsumoto Naoyuki
Faculty Of Science Kumamoto University
関連論文
- A Simple in Situ Cell-Based SUMOylation Assay with Potential Application to Drug Screening
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- A Simple in Situ Cell-Based SUMOylation Assay with Potential Application to Drug Screening
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