PCR Primers for the Neobatrachian Mitochondrial Genome
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概要
- 論文の詳細を見る
Neobatrachia is a phylogenetically-nested anuran group and contains approximately 85% of amphibian species. It is difficult to analyze the neobatrachian mitochondrial (mt) genome due to accelerated nucleotide substitution rates and occasional gene rearrangements. To overcome these problems, we designed 15 PCR primers. These primers, together with two primers from a previous study, could amplify almost the entire mt genome in all neobatrachians tested (seven species from seven different families) without optimizing PCR conditions. Among the tested taxa, Trichobatrachus robustus (Arthroleptidae) and Hyperolius viridiflavus (Hyperoliidae) showed unique amplification patterns, suggesting occurrences of gene duplication and/or rearrangements in these mt genomes.
- 日本爬虫両棲類学会の論文
著者
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Sumida Masayuki
Institute For Amphibian Biology Graduate School Of Science Hiroshima University
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KURABAYASHI Atsushi
Institute for Amphibian Biology, Hiroshima University
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Kurabayashi Atsushi
Institute For Amphibian Biology Graduate School Of Science Hiroshima University
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