Development of a Homologous Transformation System for Aspergillus aculeatus Based on the sC Gene Encoding ATP-Sulfurylase

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概要

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• A homologous transformation system was developed using the endogenous ATP-sulfurylase gene, AasC, as a selectable marker in Aspergillus aculeatus. Spontaneous mutation was proved to be beneficial in isolating AasC-deficient mutants. Molecular analysis of sC+ transformants revealed that the frequency of single copy integration at ATP-sulfurylase locus was more than 40%.

• 社団法人 日本農芸化学会の論文

著者

• KAWAGUCHI TAKASHI

  Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

• SUMITANI JUN-ICHI

  Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

• ADACHI Hiromi

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• TANI Shuji

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• KANAMASA Shin

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• Tani Shuji

  Graduate School Of Life And Environmental Sciences Osaka Prefecture University

関連論文

• Cloning and Transcription Analysis of the Aspergillus aculeatus No. F-50 Endoglucanase 2 (cmc2) Gene(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Development of a Homologous Transformation System for Aspergillus aculeatus Based on the sC Gene Encoding ATP-Sulfurylase
• Development of a Homologous Transformation System for Aspergillus aculeatus Based on the sC Gene Encoding ATP-Sulfurylase
• Transformation of Aspergillus aculeatus Using the Drug Resistance Gene of Aspergillus oryzae and the pyrG Gene of Aspergillus nidulans(Microbiology & Fermentation Technology)
• Overexpression of Aspergillus aculeatus Cellobiohydrolase I in Aspergillus oryzae(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Overexpression and Purification of Aspergillus aculeatus β-Mannosidase and Analysis of the Integrated Gene in Aspergillus oryzae

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