Expression, Purification, and Characterization of the Recombinant Putative Periplasmic Hemin-Binding Protein (HutB) of Photobacterium damselae subsp. piscicida
スポンサーリンク
概要
- 論文の詳細を見る
HutB, the periplasmic hemin binding protein of Photobacterium damselae subsp. piscicida, was produced as a recombinant protein. UV-Vis spectrophotometrical analysis showed absorption spectral changes in hemin upon mixing it with the recombinant protein, indicating complex formation. Spectrophotometric titration of HutB with hemin showed saturation at a heme/HutB ratio of 1:1 and a binding affinity (Kd) of 10 μM.
- 社団法人 日本農芸化学会の論文
著者
-
Andreoni Francesca
Department of Biomolecular Science, Section of Biotechnology, University of Urbino “Carlo Bo,” via T
-
Boiani Romina
Department of Biomolecular Science, Section of Biotechnology, University of Urbino “Carlo Bo,” via T
-
Serafini Giordano
Department of Biomolecular Science, Section of Biochemistry and Molecular Biology, University of Urb
-
BIANCONI Irene
Department of Biomolecular Science, Section of Biotechnology, University of Urbino “Carlo Bo,” via T
-
DOMINICI Sabrina
Department of Biomolecular Science, Section of Biochemistry and Molecular Biology, University of Urb
-
GORINI Francesca
Department of Biomolecular Science, Section of Biotechnology, University of Urbino “Carlo Bo,” via T
-
MAGNANI Mauro
Department of Biomolecular Science, Section of Biotechnology, University of Urbino “Carlo Bo,” via T
関連論文
- Expression, Purification, and Characterization of the Recombinant Putative Periplasmic Hemin-Binding Protein (HutB) of Photobacterium damselae subsp. piscicida
- Expression, Purification, and Characterization of the Recombinant Putative Periplasmic Hemin-Binding Protein (HutB) of Photobacterium damselae subsp. piscicida