Expression, Purification, and Characterization of the Recombinant Putative Periplasmic Hemin-Binding Protein (HutB) of Photobacterium damselae subsp. piscicida
スポンサーリンク
概要
- 論文の詳細を見る
HutB, the periplasmic hemin binding protein of Photobacterium damselae subsp. piscicida, was produced as a recombinant protein. UV-Vis spectrophotometrical analysis showed absorption spectral changes in hemin upon mixing it with the recombinant protein, indicating complex formation. Spectrophotometric titration of HutB with hemin showed saturation at a heme/HutB ratio of 1:1 and a binding affinity (Kd) of 10 μM.
- 社団法人 日本農芸化学会の論文
- 2009-05-23
著者
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Andreoni Francesca
Department of Biomolecular Science, Section of Biotechnology, University of Urbino “Carlo Bo,” via T
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DOMINICI Sabrina
Department of Biomolecular Science, Section of Biochemistry and Molecular Biology, University of Urb
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GORINI Francesca
Department of Biomolecular Science, Section of Biotechnology, University of Urbino “Carlo Bo,” via T
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Boiani Romina
Department Of Biomolecular Science Section Of Biotechnology University Of Urbino "carlo Bo"
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Magnani Mauro
Department Of Biomolecular Science Section Of Biotechnology University Of Urbino "carlo Bo"
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Bianconi Irene
Department Of Biomolecular Science Section Of Biotechnology University Of Urbino "carlo Bo"
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Serafini Giordano
Department Of Biomolecular Science Section Of Biochemistry And Molecular Biology University Of Urbin
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Gorini Francesca
Department Of Biomolecular Science Section Of Biotechnology University Of Urbino "carlo Bo"
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Dominici Sabrina
Department Of Biomolecular Science Section Of Biochemistry And Molecular Biology University Of Urbin
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Andreoni Francesca
Department Of Biomolecular Science Section Of Biotechnology University Of Urbino "carlo Bo"
関連論文
- Expression, Purification, and Characterization of the Recombinant Putative Periplasmic Hemin-Binding Protein (HutB) of Photobacterium damselae subsp. piscicida
- Expression, Purification, and Characterization of the Recombinant Putative Periplasmic Hemin-Binding Protein (HutB) of Photobacterium damselae subsp. piscicida