腱板断裂由来肩峰下滑液包線維芽細胞に対するヒアルロン酸の影響

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There is a growing body of evidence supporting the use of intra-articular hyaluronic acid (HA) injection in patients with rotator cuff disease. However, the mechanism of its anti-inflammatory action has yet to be clarified. This study was undertaken to examine the effects of HA on the expression of mRNAs for proinflammatory cytokines (IL-1 β, IL-6, and TNF- α) and COX-2/PGE2 production in IL-1-stimulated subacromial-synovium fibroblasts (SSF) derived from patients with rotator cuff disease. Various concentrations of HA were added to monolayer cultures of SSF in the presence of IL-1 β. Gene expression levels were analyzed by quantitative real-time reverse transcription (RT)-polymerase chain reaction (PCR). Intracellular production of COX-2 protein was identified by western blotting. PGE2 concentrations in the culture media were measured by ELISA. CD44 blocking with OS/37 was performed to investigate the mechanism of action of HA. Immunofluorescence cytochemistry confirmed the binding of HA and the presence of CD44 on SSF. Exogenous HA significantly and dose-dependently decreased the expression of proinflammatory cytokine mRNAs and COX-2/PGE2 production in IL-1-stimulated SSF. Pretreatment with OS/37 reversed the inhibitory effects of HA on expressions of proinflammatory cytokine mRNAs and COX-2/PGE2 production in IL-1-stimulated SSF. HA inhibited the expression of proinflammatory cytokine mRNAs and COX-2/PGE2 production via CD44 in IL-1-stimulated SSF derived from patients with rotator cuff disease. These results provide a basis for explaining why HA is effective for the treatment of rotator cuff disease.

腱板断裂由来肩峰下滑液包線維芽細胞に対するヒアルロン酸の影響

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