ヒアルロン酸の肩腱板断裂由来腱線維芽細胞への影響：細胞増殖活性とコラーゲン合成系に関して

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Hyaluronan (HA) improves postoperative recovery after flexor tendon surgery, preventing postoperative adhesion. However, its influence on the rotator cuff tendon after cuff repair has not yet been clarified in detail. HA is likely to modulate cell proliferation and mRNA expression of procollagens α 1 (I) and α 1 (III) in tendon fibroblasts derived from rotator cuff diseases. The studys subjects were 10 patients with rotator cuff disease, with an average age of 62 years old (range 44 to 72 years old). Various concentrations of HA (1.0 ∼ 5.0 mg/ml) were added to monolayer-cultured tendon fibroblasts derived from these patients. CD44 expression on the tendon fibroblasts were evaluated by confocal microscopy using fluorescein-conjugated HA and anti-human CD44 antibody (OS/37). Cell proliferation was evaluated by recording changes in cell number. The levels of expression of procollagen α 1 (I) andα 1 (III) mRNA were measured by real-time reverse transcripts polymerase chain reaction. Immunofluorescence cytochemistry detected constitutive binding of CD44 expression on the tendon fibroblasts derived from rotator cuff disease. Treatment with various concentrations of HA significantly inhibited cell proliferation and decreased the expression level of procollagen α 1 (III) mRNA, but not that of procollagen α 1 (I) mRNA, in the tendon fibroblasts. HA modulated cell proliferation and the expression level of procollagen α 1 (III) mRNA, but not that of procollagen α 1 (I), in tendon fibroblasts derived from rotator cuff disease. Postoperative use of exogenous HA may allow the healing of a repaired rotator cuff tendon with minimal adhesion.
日本肩関節学会の論文

ヒアルロン酸の肩腱板断裂由来腱線維芽細胞への影響：細胞増殖活性とコラーゲン合成系に関して

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