Cysteine-390 is the binding site of luminous substance with symplectin, a photoprotein from Okinawan squid, Symplectoteuthis oualaniensis

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概要

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• Symplectin is a photoprotein from a luminous squid, Symplectoteuthis oualaniensis. It has a luminous substrate, dehydrocoelenterazine (DCZ), linked through a thioether bond with a cysteine residue. We have proven the binding site of luminous substrate in symplectin by using an artificial analogue of DCZ, ortho-fluoro-DCZ (F-DCZ). F-DCZ-symplectin emitting strong blue light was reconstituted from apo-symplectin and F-DCZ. Proteolytic digestion of the reconstituted F-DCZ-symplectin afforded peptides including C390GLK-F-DCZ (amide), which was detected with a house assembled nano-LC-ESI-Q-TOF-MS. The chromo-peptide derived from the F-DCZ-symplectin after luminescence showed the lower molecular mass than that before the luminescence by 12 mass units, corresponding to the loss of one carbon atom upon emitting light. Thus, we have concluded that F-DCZ analogue binds to Cys390 in symplectin so as to emit light.(Edited by Teruhiko BEPPU, M.J.A.)

• 日本学士院の論文

著者

• KUSE Masaki

  Chemical Instrument Division, Research Center for Materials Science, Nagoya University

• TANI Naoki

  Laboratory of Organic Chemistry, Graduate School of Bioagricultural Sciences, Nagoya University

• ISOBE Minoru

  Institute for Advanced Research, Nagoya University

• Isobe Minoru

  Institute For Advanced Research Nagoya University

• Tani Naoki

  Laboratory Of Organic Chemistry Graduate School Of Bioagricultural Sciences Nagoya University

• Tani Naoki

  Laboratory Of Organic Chemistry Faculty Of Engineering Kanagawa University

• Kuse Masaki

  Chemical Instrument Division Research Center For Materials Science Nagoya University

• FUJII Tatsuya

  Laboratory of Organic Chemistry, Graduate School of Bioagricultural Sciences, Nagoya University

• MATSUDA Tsukasa

  Laboratory of Molecular Bioregulation, Graduate School of Bioagricultural Sciences, Nagoya Universit

• Tani Naoki

  Lab. Of Organic Chemistry Graduate School Of Bioagricultural Sciences Nagoya Univ.

• MATSUDA Tsukasa

  Laboratory of Molecular Bioregulation, Graduate School of Bioagricultural Sciences, Nagoya University

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