Differentiation of Coelenteramide Sulfates from Phosphates by Hydrogen/Deuterium Exchange Coupled with Ion Trap Mass Spectrometry
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概要
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In general, it is difficult to distinguish between sulfate and phosphate groups in natural products, because these groups are found only in trace amounts in natural products. We have devised a hydrogen/deuterium (H/D) exchange method using the liquid chromatography mass spectrometry technique for establishing a general methodology to distinguish between sulfated and phosphorylated compounds. Sulfate- or phosphate-containing compounds were differentiated by the H/D exchange method based on the number of exchangeable hydrogen in deuterated sulfate (-SO3D) and phosphate (-PO3D2), respectively. In this paper, we describe an application of this method to a natural product and in particular to Watasenia oxyluciferin that is found in the firefly squids (Hotaru-ika in Japanese) for the purpose of identifying coelenteramide disulfate.
- 2009-04-01
著者
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Nakashima Yosuke
Laboratory Of Organic Chemistry Graduate School Of Bioagricultural Sciences Nagoya University
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DOI Issei
Laboratory of Organic Chemistry, Graduate School of Bioagricultural Sciences, Nagoya University
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KUSE Masaki
Chemical Instrument Division, Research Center for Materials Science, Nagoya University
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TANI Naoki
Laboratory of Organic Chemistry, Graduate School of Bioagricultural Sciences, Nagoya University
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ISOBE Minoru
Institute for Advanced Research, Nagoya University
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Isobe Minoru
Institute For Advanced Research Nagoya University
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Doi Issei
Laboratory Of Organic Chemistry Graduate School Of Bioagricultural Sciences Nagoya University
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Tani Naoki
Laboratory Of Organic Chemistry Graduate School Of Bioagricultural Sciences Nagoya University
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Tani Naoki
Laboratory Of Organic Chemistry Faculty Of Engineering Kanagawa University
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Kuse Masaki
Chemical Instrument Division Research Center For Materials Science Nagoya University
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Tani Naoki
Lab. Of Organic Chemistry Graduate School Of Bioagricultural Sciences Nagoya Univ.
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