Beat Dependent Alteration of Ca2+-Activated Cl- Current during Rapid Stimulation in Rabbit Ventricular Myocyies.
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概要
- 論文の詳細を見る
The transient outward currents (Ito) play an important role in action potential repolarization in cardiac myocytes. Two components of Ito have been identified as 4-AP-sensitive but Ca2+-insensitive Ito carried by K+, and Ca2+-sensitive but 4-AP insensitive Ito carried by Cl- (ICl (Ca)). It is known that the amplitudes of Ito change depending on the stimulation frequency. In this study we investigated the beat dependent alteration of ICl (Ca) during rapid stimulation using the whole cell patch clamp technique in rabbit ventricular myocytes. The cells were internally perfused with a solution containing 0.1 mM free Ca2+ to develop ICl (Ca) and all internal K+ was replaced with Cs+ to block 4-AP-sensitive Ito and other K+ currents. By applying depolarizing pulses at a high frequency of 2.5 Hz, the amplitudes of ICl (Ca) gradually increased as the number of pulses increased following a transient decrease in the 2nd pulse and reached a plateau level at the 20th pulse. The shape of the current-voltage curve of ICl (Ca) was not overly different for different numbers of preceding pulses. The recovery from inactivation of ICl (Ca)could be fitted to a single expon-ential curve and full recovery was achieved after > 1 sec with a time constant of 368 ms. The ramp clamp experiments showed that the conductance of the background ICl (Ca) increased with the preceding pulse numbers, indicating that the resting level of [Ca2+]i increased with the pulses applied. From these results, we conclude that beat dependent alteration of ICl (Ca) is determined by not only its apparent kinetic property, but also the resting level of [Ca2+]i during rapid stimulation.
- International Heart Journal刊行会の論文
著者
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Kawano Seiko
Department Of Cardilvascular Disease Medical Research Institute Tokyo Medical And Dental University
-
Hiraoka Masayasu
Department Of Cardilvascular Disease Medical Research Institute Tokyo Medical And Dental University
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Kawano Seiko
Department of Cardiovascular Diseases, Tokyo Medical and Dental University
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Hirayama Yoshiyuki
Medical Research Institute, Tokyo Medical and Dental University
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Kuruma Akinori
Tokyo Medical and Dental University
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