Identification of Regulated Genes in Rat Heart after Myocardial Infarction by Means of Differential mRNA Display
スポンサーリンク
概要
- 論文の詳細を見る
In order to testify the hypothesis that unknown mechanisms are involved in the process of cardiac remodeling after myocardial infarction (MI), we employed differential display reverse transcription-polymerase chain reaction (DDRT-PCR) as our primary inspection tool. An animal model of MI was established by ligation of the left anterior descending coronary artery (LAD) in rat. Fifty upregulated candidate cDNA fragments were obtained in the right ventricle (RV) of the heart six weeks after MI. Eight cDNA fragments isolated from DD denaturing gel were extracted and reamplified, cloned into pCR II vector and sequenced. A Genbank search of these clones showed that three of them have a high homology with known genes not previously associated with cardiac remodeling, i.e., mouse interleukin-4 receptor gene, rat ferritin mRNA, and T-cell receptor beta chain V beta 5. The remaining clones have no similarity to known sequences. These data suggest that certain genes which were not previously being associated with cardiac hypertrophy are turned on during the process of cardiac remodeling after MI. (Jpn Heart J 2000; 41: 59-66)
著者
-
Unger Thomas
Department Of Pharmacology Christian-albrechts University Of Kiel Hospitalstrabe4 24105 Kiel And Ger
-
ZHU Yi-Chun
Department of Physiology, Shanghai Medical University
-
UNGER Thomas
Department of Pharmacology, Christian-Albrechts University of Kiel, 24105 Kiel, Germany.
-
Zhun ZHU
Department of Pharmacology, Christian-Albrechts University of Kiel, 24105 Kiel, Germany.
-
STOLL Monika
Department of Pharmacology, Christian-Albrechts University of Kiel, 24105 Kiel, Germany.
-
ZHU Yi-Chun
Department of Pharmacology, Christian-Albrechts University of Kiel, 24105 Kiel, Germany.
関連論文
- Time-Dependent Apoptotic Development and Pro-apoptotic Genes Expression in Rat Heart After Myocardial Infarction
- Contribution of Bradykinin to the Cardioprotective Action of Angiotensin Converting Enzyme Inhibition in Hypertension and After Myocardial Infarction
- Identification of Regulated Genes in Rat Heart after Myocardial Infarction by Means of Differential mRNA Display