High-throughput functional screening of plant and pathogen genes in planta
スポンサーリンク
概要
- 論文の詳細を見る
Understanding of molecular mechanisms of pathogenicity of Agrobacterium and plant viruses has allowed us to utilize these pathogens as efficient vectors for transgene expression in plants. These vectors are now employed for high-throughput overexpression screening of plant and pathogen genes in planta. Virus-induced gene silencing (VIGS) has become one of indispensable tools for functional analysis of genes. VIGS is also suitable for high-throughput screening of plant genes. In this review, we describe: (1) vector-mediated transient expression techniques; (2) high-throughput overexpression screening of pathogen genes, of which products elicit hypersensitive response (HR) on host plants; (3) high-throughput overexpression screening of plant genes that are related to HR-like cell death; and (4) high-throughput VIGS screening of plant genes necessary for HR.
- 日本植物細胞分子生物学会の論文
著者
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BERBERICH Thomas
Iwate Biotechnology Research Center
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Terauchi Ryohei
Iwate Biotechnology Research Center
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Hisam Bin
Iwate Biotechnology Research Center
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Ito Akiko
Iwate Biotechnology Research Center
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Saitoh Hiromasa
Iwate Biotechnology Research Center
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Takahashi Yoshihiro
Iwate Biotechnology Research Center
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