Micromorphology of Somatic Embryo Development of Castanopsis cuspidata (Thunb) var. sieboldii (Makino) Nakai
スポンサーリンク
概要
- 論文の詳細を見る
In vitro somatic embryogenesis of Castanopsis cuspidata var. sieboldii was achieved from immature embryo-derived callus and the morphological course of events during embryo development was evaluated by scanning electron microscopy technique (SEM). Embryogenic callus could be initiated from immature embryos and optimum induction of callus was observed on Murashige and Skoog (MS) medium supplemented with 10 μM 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 1 μM benzyl‐aminopurine (BAP). Somatic embryo development was occurred on half-strength basal MS medium supplemented with 2 μM BAP, 0.25 μM naphthaleneacetic acid (NAA). Somatic embryo maturation was accomplished using 1 μM zeatin, 0.25 μM indolebutyric acid (IBA). SEM observations indicated development of an extracellular material at an early stage of differentiation and a course of differentiation leading to development of somatic embryos.
- 日本植物細胞分子生物学会の論文
- 2004-09-01
著者
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Baba Shigeyuki
Department of Neurosurgery,Kurume Univeristy School of Medicine
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Baba Shigeyuki
Department Of Environment Science And Technology Faculty Of Agriculture University Of The Ryukyus
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Murayama Seiichi
Department Of Bio-production Faculty Of Agriculture University Of The Ryukyus
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VIDHANAARACHCHI Vijitha
Department of Environment Science and Technology, Faculty of Agriculture, University of the Ryukyus
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Vidhanaarachchi Vijitha
Department Of Environment Science And Technology Faculty Of Agriculture University Of The Ryukyus
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Baba Shigeyuki
Department 1, Optical Disc Development Division, Optical System Development Group, Broadband Network Company, SONY Corporation, 6-7-35 Kitashinagawa, Shinagawa-ku, Tokyo 141-0001, Japan
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