1年生植物Haplopappus gracilis(2n=4)の組織培養中の苗条原基の凍結保存〔英文〕
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概要
- 論文の詳細を見る
Shoot primordia of annual Haplopappus gracilis (2n=4) propagating vegetatively in vitro provided a new possibility for freeze preservation. The method of freeze preservation was as follows. Subcultured shoot primordia were precultured in an MS medium supplemented with 5% DMSO for 3 days. They were cooled at a velocity of -0.5°C/min down to -40°C in a programming freezer, and then stored in liquid nitrogen immediately. The frozen shoot primordia were thawed quickly in a water bath at 37°C, and recultured. The recovered shoot primordia were highly stable in the chromosome number and karyotype, which were the same as in the original plant and in the control shoot primordia without the freezing treatment. In comparison with a method using shoot tips, the above method is advantageous of being possible to store a large number of pieces of shoot primordia more easily, and also to mass-propagate plantlets clonally.
- 日本遺伝学会の論文
著者
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TANAKA Ryuso
Botanical Institute, Faculty of Science, Hiroshima University
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Taniguchi Kenji
Laboratory Of Plant Chromosome And Gene Stock Graduate School Of Science Hiroshima University
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ASHITANI Naoto
Botanical Institute, Faculty of Science, Hiroshima University
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MIYAGAWA Hideki
Laboratory of Plant Chromosome and Gene Stock, Faculty of Science, Hiroshima University
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田中 隆荘
Botanical Institute, Faculty of Science, Hiroshima University
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谷口 研至
Laboratory of Plant Chromosome and Gene Stock of the Faculty of Science, Hiroshima University
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芦谷 直登
Botanical Institute, Faculty of Science, Hiroshima University
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