In vitro production of stable new karyotype strains in culture lines of Crepis capillaris

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概要

• 論文の詳細を見る

• New karyotypes of Crepis capillaris were artificially produced and established as stable culture lines. Tissue-cultured masses of shoot primordia were first treated with 5 μg/ml aphidicolin for 24 h, followed with 5 μg/ml mitomycin C (MMC) for 24 h. Cytochimerism of karyotypes, which was obviously observed upon termination of the MMC treatment, became gradually reduced during the recovery period. After 60 days of recovery, most of the shoot primordia showed karyotype homogeneity. However, some of the shoot primordia still contained a few variations in karyotypes. On day 120, completely homogeneous masses of shoot primordia with a mutated karyotype were found, each of which were isolated as culture lines. A variety of reciprocal translocations can be produced by this system.

• 財団法人染色体学会の論文

著者

• Fujishige Ikuko

  Laboratory Of Plant Chromosome And Gene Stock Graduate School Of Science Hiroshima University

• Taniguchi Kenji

  Laboratory Of Plant Chromosome And Gene Stock Graduate School Of Science Hiroshima University

関連論文

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• クレピス,Crepis capillaris(2n=6),のカルスからの苗条原基誘導〔英文〕
• 組織培養苗条原基による1年生植物Halopappus gracilis(2n=4)における人為クロ-ン4倍体の誘導〔英文〕
• 振盪培養によるHaplopappus gracilis苗条原基の少細胞化〔英文〕
• In vitro production of stable new karyotype strains in culture lines of Crepis capillaris
• 1年生植物Haplopappus gracilis(2n=4)の組織培養中の苗条原基の凍結保存〔英文〕
• Maintenance and mass-propagation of aneuploid in annual haplopappus gracilis (2n=4) by shoot tip cloning.

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