体細胞雑種カルスにおける葉緑体ゲノムの分離様式に関する研究-3-雑種カルスにおける2種葉緑体ゲノムの無作為的分離〔英文〕

スポンサーリンク

概要

• 論文の詳細を見る

• In the parasexual hybrid callus between Nicotiana glauca and N. langsdorfii, the two parental types of chloroplasts separate during callus development. The system to investigate the mode of separation involves the hormone independent growth of the hybrid callus between N. glauca and N. langsdorfii as a selective marker for the parasexal hybrid and the isoelectrofocusing patterns of the large subunit (LS) of Fraction I protein as a chloroplast genetic marker. We improved this system by utilizing N. gossei as a chloroplast donor, because the isoelectrofocusing patterns of LS from N. gossei and that from N. langsdorfii can be distinguished clearly. The hormone independency which is suitable for selecting hybrids in the state of small calli was restored by transmitting the nuclear genome of N. glauca into N. gossei by means of sexual hybridization. Using the improved system, the mode of chloroplast separation was investigated in more detail. It was found that 84% of the cells in a two month old parasexual hybrid callus have only one or the other parental type of chloroplasts and 16% of the cells have two types.We calculated the rate of separation using the formulae based on the assumption that the two types of chloroplasts separate at random by cell divisions in the callus. The result obtained by the calculation turned out to be consistent to the experimental data obtained by the analysis of the two month old parasexual hybrid callus, showing that our assumption is reasonable.

• 日本遺伝学会の論文

著者

• 平井 篤志

  Graduate Division of Biochemical Regulation, School of Agricultural Sciences, Nagoya University

• Hirai Atsushi

  Graduate Division of Biochemical Regulation, Faculty of Agriculture, Nagoya University

• AKADA Shinji

  Graduate Division of Biochemical Regulation. Faculty of Agriculture, Nagoya University

• Hirai Atsushi

  Graduate Division Of Biochemical Regulation Faculty Of Agriculture Nagoya University

• HIRAI Atsushi

  Graduate Division of Biochemical Regulation. Faculty of Agriculture, Nagoya University

• 赤田 辰治

  Graduate Division of Biochemical Regulation, Faculty of Agriculture, Nagoya University

関連論文

• 体細胞雑種カルスのミトコンドリアDNAの多様性と分化葉におけるその均一性〔英文〕
• A Comparison of Tomato Fruit Chloroplast and Chromoplast DNAs as Analyzed with Restriction Endonucleases
• Isolation of Chloroplast DNA from Pinus
• Callus formation of somatic hybrid of rice and soybean and characteristics of the hybrid callus
• 体細胞雑種カルスにおける葉緑体ゲノムの分離様式に関する研究-3-雑種カルスにおける2種葉緑体ゲノムの無作為的分離〔英文〕
• 体細胞雑種カルスにおける葉緑体DNA種とFraction 1タンパク質の大サブユニット種の相関性〔英文〕
• 葉緑体DNAの制限酵素パタ-ンによるBrassica napusの細胞質親の検索〔英文〕

スポンサーリンク