合成オリゴタクレオチドをプロ-ブとしたキイロショウジョウバエG6PD遺伝子のクロ-ニング〔英文〕
スポンサーリンク
概要
- 論文の詳細を見る
Mixtures of 17-base long oligonucleotides possibly encoding a hexapeptide of Drosophila melanogaster glucose-6-phosphate dehydrogenase were synthesized and used as probes for screening a genomic library of D. melanogaster constructed in Charon 4A vectors. A total of about 60, 000 plaques were initially screened, and after two successive plaque purification three clones carrying the identical 13-kb EcoRI fragment were isolated. That these clones contain the G6PD coding sequence was demonstrated by in situ hybridization of the cloned DNA fragments to salivary gland polytene chromosomes and in vitro translation of the hybrid-selected mRNA. As suggested by Torczynski et al. (1984), this method using short synthetic probes appears versatile for isolating low-copy genes from genomic libraries.
- 日本遺伝学会の論文
著者
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Ito Hiroki
Department of Neurology, Ichinomiya Municipal Hospital
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Ito Hiroki
Department Of Autonomic And Behavioral Neurosciences Division Of Higher Nervous Control Institute Of
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OHTSUKA Eiko
Department of Pediatrics, Metropolitan Fuchu Medical Center for Severe Motor and Intellectual Disabi
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Hori Samuel
Department Of Zoology Faculty Of Science Hokkaido University
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Ohtsuka Eiko
Department Of Pediatrics Metropolitan Fuchu Medical Center For Severe Motor And Intellectual Disabil
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TANDA Soichi
Department of Biology, University of Maryland
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HANAOKA Takaomi
Department of Chest Surgery, Fukujuji Hospital
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AKASAKA Masami
Department of Zoology, Faculty of Science Hokkaido University
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MIURA Kazunobu
Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Science Hokkaido University
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TAKAHASHI Takayuki
Oklahoma Medical Research Foundation
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TANG J.
Oklahoma Medical Research Foundation
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Hanaoka Takaomi
Department Of Chest Surgery Fukujuji Hospital
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堀 浩
Department of Zoology, Faculty of Science, Hokkaido University
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TANDA Soichi
Department of Zoology, Faculty of Science Hokkaido University
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HORI Samuel
Department of Zoology, Faculty of Science Hokkaido University
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ITO Hiroki
Department of Advanced Materials Chemistry, Graduate School of Engineering, Yokohama National University
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ITO Hiroki
Department of Zoology, Faculty of Science Hokkaido University
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