A histochemical method to identify the genotype of single embryo cultures of Drosophila melanogaster.

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概要

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• In the developmental analyses of Drosophila mutants, it is necessary to distinguish mutant embryos from heterozygous or normal ones.We have developed an enzyme marker system by means of an X-linked mutation Zwischenfermentn (Zwn). By histochemical staining for the activity of glucose- 6-phosphate dehydrogenase (G6PD, E.C.1.1.1.49) in a single embryo culture, Zwn cultures were distinguishable from Zw+ ones.The applicability of the method to the analysis of the X-linked developmental lethals was demonstrated by examining the cell differentiation in vitro of an embryonic lethal mutation l(1)EI43. A morphological abnormality was found specifically in the cultures from hemizygous lethal embryos.

• 日本遺伝学会の論文

著者

• Miyake Tadashi

  Laboratory Of Cell Biology Mitsubishi Kasei Institute Of Life Sciences

• KOANA Takao

  Department of Biology, Division of Natural Sciences, International Christian University

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• A histochemical method to identify the genotype of single embryo cultures of Drosophila melanogaster.
• Insertional mutagenesis in Drosophila. I Efficiency of transformation and induction of mutations in Drosophila melanogaster.:I. Efficiency of transformation and induction of mutations in Drosophila melanogaster

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