体細胞突然変異の研究-1-培養されたチャイニ-ズハムスタ-hai細胞における放射線および化学物質による栄養要求性ならびに非要求性突然変異の誘発〔英文〕

概要

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A forward mutation system using prototrophic CH-hai Cl 23 cells (Ala+, Asn+, Pro+, Asp+, Hyp+, Glu+) isolated from an original Chinese hamster hai cell line was found to be much more sensitive than a reverse mutation system using auxotrophic CH-hai Cl 3 cells (TdR-), for detecting the mutations induced by chemical carcinogens or mutagens (4-nitroquinoline 1-oxide, N-methyl-Nnitro-N-nitrosoguanidine, ethyl methane-sulfonate), as well as by X-rays and UV. It was found that the antimicrobial food additive, 2-(2-furyl)-3-(5-nitro-2-furyl) acrylic acid amide (furylfuramide: FF or AF2) has highly mutagenic activity so far as it was tested by the forward mutations. The forward mutation system, from prototrophic CH-hai Cl 23 cells to auxotrophs, established in this study can be applied as a useful system for detecting environmental mutagens and for elucidating the mechanisms of somatic cell mutation.
日本遺伝学会の論文