ISOLATION AND CHARACTERIZATION OF NUTRITIONALLY DEFICIENT CELLS FROM CULTURED CHINESE HAMSTER HAI CELLS BY A REPLICA PLATING METHOD
スポンサーリンク
概要
- 論文の詳細を見る
Nutritionally deficient cells were detected and isolated from cultures of Chinese hamster hai cells by our replica plating method. The nutritionally sufficient characteristics (Ala+, Asn+, Pro+, Asp+, Hyp+) of a prototrophic clone isolated in the first survey, which was performed about one month after the cells were transferred to the complete medium from the original medium, were stable. On the other hand, the majority among the nutritionally deficient characteristics (Ala-, Asn-, Pro-, Ser-, Gly-, TdR-) of an auxotrophic clone isolated in the second survey, which was performed about one year after the cells were transferred to the complete medium, were unstable. Only the thymidine-deficient characteristics of this clone were stable, and the mean reverse mutation frequency of this characteristic was 4.7×10-6. These results indicate that epigenetic as well as genetic changes may be related to the changes in nutritional requirement of mammalian cells and that our replica plating method might equally detect the cells having apparently genetic characteristics altered by epigenetic as well as genetic changes.
- 日本遺伝学会の論文
著者
-
Suzuki Fumio
Division Of Cardiology Arrhythmia Institute Tokyo Heart Center
-
HORIKAWA MASAKATSU
Division of Radiation Biology, Faculty of Pharmaceutical Sciences, Kanazawa University
関連論文
- PE-112 Catheter Mapping of Rightward and Leftward Posterior AV Nodal Extensions in Patients with Demonstrable Retrograde Dual AV Nodal Pathways(Arrhythmia, diagnosis/pathophysiology/EPS-08, The 71st Annual Scientific Meeting of the Japanese Circulation So
- OJ-219 Abrupt Disappearance of Large Atrial Potentials at the Distal Coronary Sinus (CS) Strongly Suggests Loss of CS Musculature(Arrhythmia, Diagnosis/Pathophysiology/EPS 8 (A) : OJ25)(Oral Presentation (Japanese))
- A Specific Chromosome Change and distinctive Transforming Genes Are Necessary for Malignant Progression of Spontaneous Transformation in Cultured Chinese Hamster Embryo Cells
- チャイニ-ズハムスタ-細胞における放射線誘発致死障害および突然変異障害の回復〔英文〕
- 体細胞突然変異の研究-1-培養されたチャイニ-ズハムスタ-hai細胞における放射線および化学物質による栄養要求性ならびに非要求性突然変異の誘発〔英文〕
- Analysis of Transforming Genes in Indirectly Induced Radiogenic Thymomas in Mice
- Electrical Connections between Persistent Left Superior Vena Cava and Left Atrium during Catheter Ablation for Atrial Tachycardia
- 培養されたヒト由来細胞株(XP細胞とHeLaS3細胞)におけるX線誘発細胞死と突然変異〔英文〕
- 同調培養されたチャイニーズハムスターDon細胞から分裂中期染色体の分離〔英文〕
- Modes of Initiation of Two Types of Atrial Reentry in a Patient with Typical Atrial Flutter: Isthmus-dependent Micro-reentry versus Macro-reentry
- ISOLATION AND CHARACTERIZATION OF NUTRITIONALLY DEFICIENT CELLS FROM CULTURED CHINESE HAMSTER HAI CELLS BY A REPLICA PLATING METHOD
- STUDIES ON SOMATIC CELL MUTATIONS:II. RADIATION-INDUCTION OF 8-AZAGUANINE-RESISTANT AND -SENSITIVE MUTANTS IN CHINESE HAMSTER HAI CELLS IN VITRO