3倍性Brassica雑種の子房の試験管培養-2-発達途中における組織学的研究〔英文〕
スポンサーリンク
概要
- 論文の詳細を見る
Histological observation of ovary culture was made in the reciprocal crosses between diploid Brassica campestris L. ssp. chinensis (L.) Makino and autotetraploid B. campestris L. ssp. (Lour.) Olsson and their control (2x×2x and 4x×4x).The ovaries were cut from maternal plants four days after pollination and then were soon explanted aseptically in the simple and complex medium with growth regulating substances (IAA, kinetin and gibberellin), yeast extract, tomato juice, casein hydrolysate and coconut milk.Histological observation of the embryo, endosperm and seed coat was made 11 days after explantation (15 days after pollination). The results showed that the interruption of developing embryo in 4x×2x was due to poor development of the endosperm. Degeneration of embryos in 2x×4x and 4x×4x, was due to the abnormalities of endosperm and due to poorly developed embryos.The development of embryos and endosperm was little influenced by the medium with the growth regulating substances (IAA, kinetin, gibberellin) and was influenced in the medium added with yeast extract, tomato juice, casein hydrolysate and coconut milk. Tomato juice did not promote development of ovules. The yeast extract and casein hydrolysate promoted development of embryos and endosperm in the crosses of the control (2x×2x, 4x×4x) and 4x×2x. Coconut mlik promoted in the crosses of the control and 2x×4x.The embryos which developed earliest were three to seven days earlier than those under natural condition in each cross combination. When development of the ovule was later than that found in natural condition, abnormalities of embryo and endosperm were observed.
- Genetics Society of Japanの論文
著者
-
猪俣 伸道
Laboratory of Genetics, Faculty of Agriculture, Kyoto University, Kyoto
-
INOMATA NOBUMICHI
Laboratory of Genetics and Plant Breeding, College of Agriculture, University of Osaka Prefecture