空気乾燥法による哺乳類染色体におけるギムザ・バンド形成の要因〔英文〕

概要

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The conditions for Giemsa-band formation in air-dried chromosomes of HeLa cells and FL cells were examined in detail. When chromosome preparations were stained immediately after air drying, Giemsa staining alone, if the staining solutions were prepared with saline solutions of which pH values were higher than 6, produced bands. It seems that a variety of pretreatments including trypsin treatment so far reported are not essential conditions for band formation but rather promoting ones. Brief refixations with formalin, glutaraldehyde or picric acid solution prior to band inducing procedures markedly prevented band formation. Basic dye itself appeared to act to prevent band formation, since chromosomes once stained with Giemsa or toluidine blue became extremely reluctant to show bands. It seems very likely that the structural lesion of chromosomal porteins would be an indispensable triggar to induce banded structures.
日本遺伝学会の論文