ヒゲカビ(Phycomyces)における外来遺伝子導入法の開発〔英文〕
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概要
- 論文の詳細を見る
An efficient method is described for obtaining transformants in Phycomyces by microinjection of plasmid DNA carrying the kanamycin resistance gene from Tn903 into a young sporangium. Approximately 9% of the colonies, germinated from the spores which were developed in the sporangium, were resistant to G418 when a 36 ng sample of the transforming plasmid DNA was injected into a sporangium. The transformation efficiency (transfomants/total colonies) was about 350 times higher than that (transfomants/total protoplasts) obtained with the ordinary protoplast method.
- 日本遺伝学会の論文
著者
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MIYAZAKI Atsushi
Institute of Astrophysics and Planetary Science, Ibaraki University
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OOTAKI Tamotsu
Institute of Genetic Ecology, Tohoku University
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FUKUI Joe
Institute of Genetic Ecology, Tohoku University
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Ootaki Tamotsu
Institute Of Genetic Ecology Tohoku University
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KIMURA Yuji
Faculty of Science, Yamagata University
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UMEMURA Seiichi
Faculty of Science, Yamagata University
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OKAZAKI Ryoko
Faculty of Education, Yamagata University
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JAYARAM Makkuni
Department of Microbiology, The University of Texas at Austin
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大滝 保
Institute of Genetic Ecology, Tohoku University
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宮嵜 厚
Institute of Genetic Ecology, Tohoku University
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福井 丈
Institute of Genetic Ecology, Tohoku University
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- ヒゲカビ(Phycomyces)における外来遺伝子導入法の開発〔英文〕