Embryological genetics on the resistance to parathion in Drosophila melanogaster:II. Resistance to parathion of various larval organs in tissue culture
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1. The eye discs, the wing discs, the cephalic complexes, the leg discs, the haltere discs, and the salivary glands of the mature third-instar larvae (about 96 hours after hatching) of the insecticide-resistant strains (Hikone and WMB) and the insecticide-susceptible strains (Fukuoka and Canton-S) of D. melanogaster were cultured in the synthetic media containing 25.0ppm and 50.0ppm parathion respectively.2. When 25.0ppm parathion was added to the medium, it was observed after 24 hours culture that the eye discs, the wing discs, the cephalic complexes, and the leg discs of Fukuoka and Canton-S strains were more pronouncedly inhibited than those of Hikone and WMB strains. The haltere discs of all strains were markedly inhibited in the medium containing 25.0ppm parathion. The salivary glands of all strains were less inhibited by 25.0ppm parathion.3. When 50.0ppm parathion was added to the medium, every organs of the insecticide-resistant strains and of the insecticide-susceptible ones were pronouncedly inhibited after culturing for 24 hours.
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- Embryological genetics on the resistance to parathion in Drosophila melanogaster:II. Resistance to parathion of various larval organs in tissue culture