Extraction of Phospholipids from Unused Natural Resources with Supercritical Carbon Dioxide and an Entrainer

概要

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The authors focus on tuna shavings as a natural resource which hither to has been unused, they contain a high concentration of phospholipids including docosahexaenoic acid (DHA-PL) and are a by-product of factory processed tuna. Supercritical carbon dioxide (SC-CO2) although a suitable substance in the extraction of non-polar substances (triacylglycerides), has not proven effective in the extraction of polar substances. The efficient use of SC-CO2/ethanol to extract and fractionate phospholipids from tuna shavings was therefore investigated. Extraction was performed at low pressure and temperature (17.7 MPa and 33°C) to avoid oxidation of polyunsaturated fatty acids. Phospholipids were extracted and fractionated with more than 5%-ethanol in SC-CO2. To reduce the amount of consumed ethanol is an important problem to overcome in extracting on an industrial scale. The tuna shavings were soaked in the same volume of ethanol before extraction with neat SC-CO2. For performance on an industrial scale, the extracting conditions were evaluated using a 10 mL-laboratory scale reactor. 182.8 kg of soaked tuna shavings was finally extracted and 4.2 kg of DHA-PL (DHA content = 29%) was obtained in a series of industrial processes.
日本油化学会の論文