Preparation of Ethyl Magnesium Bromide for Regiospecific Analysis of Triacylglycerols

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概要

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• This paper presents a procedure for preparation of a Grignard reagent, ethyl magnesium bromide, used for partial deacylation of triacylglycerols (TAG) in their regiospecific analysis. Magnesium turnings were reacted with ethereal solution of bromoethane in a screw-capped test tube to synthesize 2 mL of 1 M ethyl magnesium bromide. Continuously stirred with a vortex mixer, the reaction smoothly proceeded at room temperature. Regiospecific analysis of 1,3-distearoyl-2-oleoylglycerol using this product showed that fatty acid compositions of the sn-1(3) and sn-2 positions were contaminated by less than 2 mol% of fatty acids migrated from isomeric positions. The analyses of lard and cod liver/mackerel oil TAG showed typical distribution patterns of 16:0, 22:5n-3 and 22:6n-3 in pig and fish depot TAG. These results confirmed the view that the freshly prepared reagent is usable for regiospecific analysis of TAG.

• 日本油化学会の論文

著者

• Ando Yasuhiro

  Laboratory Of Bioresources Chemistry Division Of Marine Biosciences Graduate School Of Fisheries Sci

• Tomita Yuki

  School of Fisheries Sciences, Hokkaido University

• Haba Yusuke

  Graduate School of Fisheries Sciences, Hokkaido University

• Haba Yusuke

  Graduate School Of Fisheries Sciences Hokkaido Univ.

• Tomita Yuki

  School of Engineering, the University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan

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• Distribution of Novel Nonmethylene-Interrupted Fatty Acids over Neutral and Polar Lipids of Ophiuroidea (Brittle Star)
• Preparation of Ethyl Magnesium Bromide for Regiospecific Analysis of Triacylglycerols
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