Expression of mRNA for Growth Factor Receptors in Porcine Cumulus-Oocyte Complexes during In Vitro Maturation

概要

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The information concerning the transcription of growth factor receptor mRNA in porcine cumulus-oocytes complexes (COCs) during oocyte maturation is limited. The expression of mRNA was analyzed for growth factor receptors with receptor tyrosine kinases [epidermal growth factor receptor (EGF-R), ErbB3 (a member of EGF-R subfamily), insulin-like growth factor-I receptor (IGF-I-R), basic fibroblast growth factor receptor (bFGF-R) and platelet-derived growth factor receptor α (PDGF-Rα)] by reverse transcription-polymerase chain reaction in porcine COCs during oocyte maturation in vitro. Porcine oocytes or their surrounding cumulus cells were analyzed immediately after collection (0 h), at 12, 24, 36 and 42 h of in vitro maturation, respectively. Whereas EGF-R transcript was not observed in both oocytes and cumulus cells throughout in vitro maturation, ErbB3, IGF-I-R, bFGF-R and PDGF-Rα mRNAs were detectable in both oocytes and cumulus cells at all stages of in vitro maturation. The intensity of bands for ErbB3, IGF-I-R, and bFGF-R transcripts in oocytes was higher than that for PDGF-Rα transcript in oocytes at all stages of in vitro maturation. These results provide evidence that several growth factor receptor mRNAs are present in both oocytes and cumulus cells during in vitro maturation, and support the concept that those factors are involved in the regulation of porcine oocyte maturation.
日本繁殖生物学会の論文