Enhancement of Cytoplasmic Maturation of In Vitro-Matured Pig Oocytes by Mechanical Vibration
スポンサーリンク
概要
- 論文の詳細を見る
The effects of mechanical vibration during in vitro maturation and/or in vitro culture after artificial activation of pig oocytes on maturation and development were examined. In addition, the optimal conditions were applied to in vitro production of blastocysts derived from miniature pig somatic cell nuclear transfer (SCNT) embryos. Mechanical vibration during in vitro maturation did not affect the rates (60.5 ± 1.9-69.5 ± 2.2%) of oocytes reaching the metaphase-II stage. However, the blastocyst formation rates after activation of oocytes matured with mechanical vibration for 5 sec at intervals of 30-60 min or for 10 sec at intervals of 60 min were significantly (P<0.05) higher than those of oocytes matured without mechanical vibration (25.7 ± 2.0-28.1 ± 2.7% vs. 12.3 ± 1.4% and 25.8 ± 1.8% vs. 15.7 ± 1.9%, respectively). In contrast, mechanical vibration during in vitro culture after activation did not affect the blastocyst formation (11.6 ± 5.2-16.5 ± 3.0%) of oocytes. Mechanical vibration for 5 sec at intervals of 60 min during in vitro maturation of oocytes did not affect fusion (66.8 ± 3.5-72.1 ± 3.1%) with miniature pig somatic cells after enucleation. However, the blastocyst formation rate of SCNT embryos was improved (P<0.05) by mechanically vibrating recipient oocytes for 5 sec at intervals of 60 min during in vitro maturation, regardless of the presence or absence of the same treatment during in vitro culture (17.6 ± 2.5% vs. 9.4 ± 0.9% and 13.0 ± 0.3% vs. 7.4 ± 0.9%, respectively). The results indicated that mechanical vibration enhances the cytoplasmic maturation of in vitro-matured pig oocytes, resulting in improvement of their parthenogenetic development. In addition, it was shown that in vitro maturation of oocytes with mechanical vibration can be applied to efficient production of blastocysts derived from miniature pig SCNT embryos.
- 2010-04-01
著者
-
MIZOBE Yamato
Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University
-
YOSHIDA Mitsutoshi
Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University
-
MIYOSHI Kazuchika
Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University
-
Miyoshi Kazuchika
Laboratory Of Animal Reproduction Faculty Of Agriculture Kagoshima University
-
Miyoshi Kazuchika
鹿児島大学 農学部家畜繁殖学
-
Yoshida Mitsutoshi
Laboratory Of Animal Reproduction Faculty Of Agriculture Kagoshima University
-
Yoshida Mitsutoshi
鹿児島大学 農学部家畜繁殖学
-
Mizobe Yamato
Laboratory Of Animal Reproduction Faculty Of Agriculture Kagoshima University
関連論文
- Production of Genetically Modified Porcine Blastocysts by Somatic Cell Nuclear Transfer: Preliminary Results Toward Production of Xenograft-Competent Miniature Pigs
- Enhancement of Cytoplasmic Maturation of In Vitro-Matured Pig Oocytes by Mechanical Vibration
- Beneficial Effects of Reversine on In Vitro Development of Miniature Pig Somatic Cell Nuclear Transfer Embryos
- Usefulness of a Non-invasive Reporter System for Monitoring Reprogramming State in Pig Cells: Results of a Cell Fusion Experiment
- Production of genetically modified porcine blastocysts by somatic cell nuclear transfer: preliminary results toward production of xenograft-competent miniature pigs
- Usefulness of a Non-invasive Reporter System for Monitoring Reprogramming State in Pig Cells : Results of a Cell Fusion Experiment
- ミニブタ体細胞クローン胚の作出に用いる融合および活性化用培地中におけるカルシウム濃度の最適化
- Establishment of an In Vitro Production System for Nuclear Transfer Embryos Reconstituted with Cultured Cells in Pigs
- Development of a Noninvasive Monitoring System for Evaluation of Oct-3/4 Promoter Status in Miniature Pig Somatic Cell Nuclear Transfer Embryos
- Effects of Cycloheximide on Parthenogenetic Development of Pig Oocytes Activated by Ultrasound Treatment
- Effects of Demecolcine and Sucrose on the Incidence of Cytoplasmic Protrusions Containing Chromosomes in Pig Oocytes Matured In Vitro
- Activation and Parthenogenetic Development of Pig Oocytes Exposed to Ultrasound in Media Containing Different Concentrations of Ca^
- 胚由来細胞および卵丘細胞を用いたラットにおける核移植
- Expression of mRNA for Growth Factor Receptors in Porcine Cumulus-Oocyte Complexes during In Vitro Maturation
- Detection of Growth Factor mRNAs in Porcine Cumulus-Oocyte Complexes During Maturation In Vivo and In Vitro
- Optimization of Ca^ Concentrations in Fusion and Activation Media for Production of Cloned Embryos from Miniature Pig Somatic Cells
- Expression of mRNA for Growth Factor Receptors in Porcine Cumulus-Oocyte Complexes during In Vitro Maturation
- Detection of Growth Factor mRNAs in Porcine Cumulus-Oocyte Complexes During Maturation In Vivo and In Vitro
- Production of Genetically Modified Porcine Blastocysts by Somatic Cell Nuclear Transfer : Preliminary Results Toward Production of Xenograft-Competent Miniature Pigs
- Effects of trichostatin A on in vitro development and transgene function in somatic cell nuclear transfer embryos derived from transgenic Clawn miniature pig cells
- Stage-specific effects of osmolarity of a culture medium on development of pig oocytes and miniature pig somatic cell nuclear transfer embryos activated by ultrasound treatment
- Successful Suppression of Endogenous α-1,3-Galactosyltransferase Expression by RNA Interference in Pig Embryos Generated In Vitro
- Successful Suppression of Endogenous α-1,3-Galactosyltransferase Expression by RNA Interference in Pig Embryos Generated In Vitro
- Effect of Postactivation Treatment with Latrunculin A on In Vitro and In Vivo Development of Cloned Embryos Derived from Kidney Fibroblasts of an Aged Clawn Miniature Boar