精子形成過程でのマウスTRF2遺伝子発現

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概要

• 論文の詳細を見る

• The gene encoding TATA-binding protein-related factor 2 (TRF2/TLF/TLP/TRP), essential for the progress of spermiogenesis, is abundantly expressed in mammalian testis. A sequence database search revealed that mouse TRF2 is encoded by two mRNAs containing the same protein-coding region and different 5-untranslated regions. Northern blot analysis using DNA probes specific for the 5-untranslated regions demonstrated that these two mRNAs are distinguished from each other by the expression patterns: ubiquitous and testis-specific expression. The ubiquitously expressed form of TRF2 mRNA was present at a very low level throughout testicular development, whereas expression of the testis-specific form was first detectable in the 14-day-old testis, and the mRNA level abundantly increased at the later stages of testicular development. Western blot analysis indicated that the TRF2 level increases during testicular development, which is consistent with the expression pattern of the testicular form of TRF2 mRNA. Thus, the presence of the testis-specific form of TRF2 mRNA may account for overexpression of the TRF2 gene in the testis.

• 日本繁殖生物学会の論文

著者

• 柏原 真一

  筑波大学 大学院生命環境科学研究科・応用生物化学系

• 柏原 真一

  Institute of Applied Biochemistry, University of Tsukuba

• BABA Tadashi

  Institute of Applied Biochemistry, University of Tsukuba

• 杉浦 伸

  Institute of Applied Biochemistry, University of Tsukuba

• 岩瀬 茂樹

  Institute of Applied Biochemistry, University of Tsukuba

• SUGIURA Shin

  Institute of Applied Biochemistry, University of Tsukuba

• KASHIWABARA Shin-ichi

  Institute of Applied Biochemistry, University of Tsukuba

• IWASE Shigeki

  Institute of Applied Biochemistry, University of Tsukuba

• Baba Tadashi

  Institute Of Applied Biochemistry University Of Tsukuba

• Kashiwabara Shin-ichi

  Institute Of Applied Biochemistry University Of Tsukuba

関連論文

• 精巣特異的ポリAポリメラーゼTPAPトランスジーンの野生型とTPAP欠損マウスでの発現
• 精子形成過程でのマウスTRF2遺伝子発現
• Y染色体の大部分を欠損している自然変異マウスの伸長細胞アクロソームに出現する球状微小胞に関する研究
• Roles of the testis/sperm-specific ADAM family in sperm-egg interaction(Symposium on the Mechanism of Signal Transduction in Fertilization: its Diversity, Universality and Evolution)
• Identification of two different isoforms of mouse ADAM 1 (fertilin α)
• 精巣特異的ポリAポリメラーゼTPAPトランスジーンの野生型とTPAP欠損マウスでの発現

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