精巣特異的ポリAポリメラーゼTPAPトランスジーンの野生型とTPAP欠損マウスでの発現

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概要

• 論文の詳細を見る

• We have previously identified a testis-specific poly(A) polymerase, TPAP (PAPβ), involved in poly(A) tail extension of specific mRNAs in the cytoplasm of round spermatids. Targeted disruption of the mouse TPAP gene resulted in the arrest of spermiogenesis due to reduced expression of haploid-specific genes required for morphogenesis of germ cells. To further elucidate the role(s) of TPAP in spermatogenesis, transgenic mice expressing an exogenous TPAP transgene on wild-type and TPAP-deficient backgrounds were generated and characterized. The transgenic mice overexpressing TPAP exhibited normal spermatogenesis and fertility. The sizes of some transcription factor mRNAs as the substrates of TPAP were also unaffected. Transgenic expression of the TPAP gene in the TPAP-deficient mice complemented both the incomplete elongation of the poly(A) tails of specific transcription factor mRNAs, and reduced expression of haploid-specific genes, resulting in the resumption of normal spermiogenesis. These data conclusively show that spermatogenesis requires the cytoplasmic elongation of the mRNA poly(A) tails catalyzed by TPAP, and imply the presence of a regulatory mechanism(s) defining the extent of the cytoplasmic mRNA polyadenylation.

• 日本繁殖生物学会の論文
• 2004-04-01

著者

• Zhuang Tiangang

  Institute of Applied Biochemistry, University of Tsukuba

• 柏原 真一

  Institute of Applied Biochemistry, University of Tsukuba

• 野口 純子

  Germ Cell Conservation Laboratory, National Institute of Agrobiological Sciences

• KASHIWABARA Shin-ichi

  Institute of Applied Biochemistry, University of Tsukuba

• Noguchi Junko

  Germ Cell Conservation Laboratory National Institute Of Agrobiological Sciences

• Zhuang Tiangang

  Institute Of Applied Biochemistry University Of Tsukuba

• Baba Tadashi

  Institute Of Applied Biochemistry University Of Tsukuba

• Kashiwabara Shin-ichi

  Institute Of Applied Biochemistry University Of Tsukuba

関連論文

• 精巣特異的ポリAポリメラーゼTPAPトランスジーンの野生型とTPAP欠損マウスでの発現
• 精子形成過程でのマウスTRF2遺伝子発現
• Y染色体の大部分を欠損している自然変異マウスの伸長細胞アクロソームに出現する球状微小胞に関する研究
• Roles of the testis/sperm-specific ADAM family in sperm-egg interaction(Symposium on the Mechanism of Signal Transduction in Fertilization: its Diversity, Universality and Evolution)
• Identification of two different isoforms of mouse ADAM 1 (fertilin α)
• 精巣特異的ポリAポリメラーゼTPAPトランスジーンの野生型とTPAP欠損マウスでの発現
• Successful Long Term Culture of Immature Porcine Sertoli Cells in the Reconstructed Testicular Cell Cord

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