性ステロイド補充療法後、性周期中および妊娠初期の未経産雌ブタにおける子宮内膜のFibroblast Growth Factor 7(FGF-7)とその受容体(FGFR2IIIb)の発現制御について
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概要
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The aim of this study was to characterize the effect of ovarian steroids and early gestation on the expression of fibroblast growth factor 7 (FGF-7) and its receptor (FGFR2IIIb) in the porcine endometrium. In Experiment 1, gilts were ovariectomized (OVX) on day 10 of the estrous cycle and treated thereafter with vehicle (VEH), progesterone (P4), estradiol benzoate (EB), or P4 + EB. Days 12 and 20 cyclic gilts (C12 and C20) were used to determine the influence of physiologically low and high plasma estradiol and progesterone concentrations on their expression. In Experiment 2, the expression of FGF-7 and FGFR2IIIB was characterized on days 1 (G 1) and 12 (G 12) of gestation. FGF-7 and FGFR2IIIb mRNA were quantified by quantitative real-time RT-PCR, and localization of FGF-7 protein in steroid-treated and early pregnant gilts was performed by immunohistochemistry. VEH-gilts expressed both FGF-7 and FGFR2IIIB mRNA. We found a significant effect of EB, but no effects of P4 or P4+EB on the mRNA expression of FGF-7. FGFR2IIIb mRNA significantly decreased after the EB and combined P4 + EB treatments, compared to P4 only substituted animals. Day 12 cyclic gilts showed significantly higher FGF-7 and FGFR2IIIb mRNA expression compared with day 20 gilts. Between day 1 and 12 of gestation, FGF-7 mRNA expression differed highly while FGFR2IIIb transcripts only varied significantly. FGF-7 protein was localized in endometrial epithelia, vascular smooth muscle, and the endothelium of different types of blood vessels. Staining was weak in VEH and P4 treated gilts, whereas it was prominent following EB and P4+EB. FGF-7 antibody strongly stained the luminal epithelium on day 12 of gestation. In summary, FGF-7 and FGFR2IIIb mRNA expression is regulated differently by exogenous ovarian steroids, assuming progesterone in connection with a specific amount of 17β-estradiol, whereas the receptor seems to be inhibited by estradiol. Both transcripts coordinately increased during the progesterone dominated phase on day 12 both in cyclic and early pregnant gilts. We conclude that estradiol and progesterone are involved in the regulation of this ligand-receptor system, which might have an important role in preparing endometrial tissue for implantation in gilts.
- 日本繁殖生物学会の論文
著者
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Welter Harald
ドイツ
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BRUSSOW Klaus-Peter
Research Institute for the Biology of Farm Animals
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Wollenhaupt Karin
Research Institute for the Biology of Farm Animals, Reproductive Biology
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Welter Harald
Institute of Physiology, Technical University of Munich
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EINSPANIER Ralf
Institute of Veterinary Biochemistry, Free University of Berlin
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Bruessow K‐p
ドイツ国立デュマスドルフ家畜研究所繁殖部
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Bruessow Klaus-peter
ドイツ
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Welter Harald
Institute Of Physiology Technical University Of Munich
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Welter Harald
Institute Of Physiology Technical University Munich
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Einspanier Ralf
Institute Of Veterinary Biochemistry Free University Of Berlin
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Wollenhaupt Karin
Leibniz Inst. For Farm Animal Biology (fbn)
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BRÜSSOW Klaus-Peter
Research Institute for the Biology of Farm Animals, Reproductive Biology
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- 性ステロイド補充療法後、性周期中および妊娠初期の未経産雌ブタにおける子宮内膜のFibroblast Growth Factor 7(FGF-7)とその受容体(FGFR2IIIb)の発現制御について
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