Effect of Exposure Duration of Ovaries and Oocytes at Ambient Temperature on Parthenogenetic Development of Porcine Follicular Oocytes

スポンサーリンク

概要

• 論文の詳細を見る

• This study was conducted to evaluate the effects of exposing porcine ovaries to 30-33 C during transportation for 4 h and subsequently room temperature (25 C) for 6 h of storage on in vitro maturation (IVM) and subsequent parthenogenetic development of oocytes collected from the ovaries. After IVM, oocytes having a tight oopalsm membrane and no signs of degeneration were exposed to Dulbeccos phosphate-buffered saline (DPBS) with 7% ethanol (v/v) for 7 min to induce parthenogenetic activation. Moreover, we also determined whether exposure of the collected oocytes to room temperature for 1, 2, and 4 h in DPBS or porcine follicular fluid (pFF) affected parthenogenetic development. When porcine ovaries were stored after transportation, oocytes collected from the stored ovaries showed a significantly higher rate of degeneration after 65 h of IVM (58.4%) and a significantly lower rate of cleavage after parthenogenetic activation (40.1%) than oocytes collected from ovaries immediately after transportation (38.9% and 47.4%, respectively). However, there was no significant difference in developmental rates to the morula and blastocyst stages between these two groups (14.4% and 14.3%, respectively). The duration of preservation, 1, 2, and 4 h, of oocytes in DPBS did not affect parthenogenetic development. In contrast, when preserved for 4 h in pFF, the developmental rates of the oocytes were significantly decreased. This suggested that some factor(s) in follicular fluid affects the developmental rate of oocytes with the passage of time in ambient conditions. These results suggest that even after 6h storage of ovaries, oocytes having normal morphology after IVM have the same rate of parthenogenetic development as oocytes collected from ovaries just after 4 h of transportation, except for a lower cleavage rate, and that exposure of oocytes to room temperature for 4 h in DPBS does not affect their parthenogenetic developmental competence.

• 日本繁殖生物学会の論文
• 2006-10-01

著者

• Kim Ill-hwa

  韓国

• Cho Sang-rae

  Animal Genetic Resources Station National Institute Of Animal Science Rda

• Kim Ill-hwa

  Laboratory Of Theriogenology College Of Veterinary Medicine Chungbuk National University

• KIM Hyun-Jong

  National Livestock Research Institute, RDA

• CHOI Sun-Ho

  National Livestock Research Institute, RDA

• SON Dong-Soo

  National Livestock Research Institute, RDA

• CHO Sang-Rae

  National Livestock Research Institute, RDA

• CHOE Chang-Yong

  National Livestock Research Institute, RDA

• KIM Young-Keun

  National Livestock Research Institute, RDA

• HAN Man-Hye

  National Livestock Research Institute, RDA

• RYU Il-Sun

  National Livestock Research Institute, RDA

• KIM In-Cheul

  National Livestock Research Institute, RDA

• KIM Il-Hwa

  Department of Veterinary Medicine, Chungbuk National University

• IM Kyung-Soon

  Department of Animal Biotechnology, Seoul National University

• NAGAI Takashi

  Developmental Biology Department, National Institute of Agrobiological Sciences

• Han Man-hye

  National Livestock Research Institute Rda

• Ryu Il-sun

  National Livestock Research Institute Rda

• Son Dong-soo

  Animal Genetic Resources Station National Institute Of Animal Science Rda

• Kim Hyun-jong

  Animal Genetic Resources Station National Institute Of Animal Science Rda

• Im Kyung-soon

  Department Of Animal Biotechnology Seoul National University

• Kim Young-keun

  National Livestock Research Institute Rda

• HAN Man-Hye

  Republic of Korea Animal Genetic Resources Station, National Institute of Animal Science, RDA

• Choe Chang-yong

  Republic Of Korea Animal Genetic Resources Station National Institute Of Animal Science Rda

• Han Man-hye

  Republic Of Korea Animal Genetic Resources Station National Institute Of Animal Science Rda

• Nagai Takashi

  Developmental Biology Department National Institute Of Agrobiological Sciences

関連論文

• 分娩後子宮内膜炎発症牛の分娩前後における免疫反応
• A CIDR-Based Timed Embryo Transfer Protocol Increases the Pregnancy Rate of Lactating Repeat Breeder Dairy Cows
• The Effect of Reduced Dose and Number of Treatments of FSH on Superovulatory Response in CIDR-Treated Korean Native Cows
• Effect of Exposure Duration of Ovaries and Oocytes at Ambient Temperature on Parthenogenetic Development of Porcine Follicular Oocytes
• Intracytoplasmic Sperm Injection in the Pig : Where is the Problem?
• 韓国における乳牛の分娩後子宮内膜炎のリスクファクターと子宮内膜炎がその後の繁殖成績に及ぼす影響
• The Effects of Administering Estradiol Benzoate Plus Progesterone during the Growth or Static Phases of the Dominant Follicle in CIDR-Treated Lactating Dairy Cows
• Synergistic Effects of Glutathione and β-mercaptoethanol Treatment During In Vitro Maturation of Porcine Oocytes on Early Embryonic Development in a Culture System Supplemented with L-cysteine
• Synergistic Effects of Glutathione and β-Mercaptoethanol Treatment During In Vitro Maturation of Porcine Oocytes on Early Embryonic Development in a Culture System Supplemented with L-cysteine
• Immune Responses during the Peripartum Period in Dairy Cows with Postpartum Endometritis
• Comparison of Two Types of CIDR-based Timed Artificial Insemination Protocols for Repeat Breeder Dairy Cows

もっと見る 閉じる

スポンサーリンク