Effect of Culture Medium and Prostaglandin I_2 (PGI_2) Analogue on In Vitro Development of Parthenogenetically Activated Cat Oocytes

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概要

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• A method of reliably producing developmentally competent cat embryos in vitro is a prerequisite for study of the physiology of early development and application of assisted reproductive techniques. Oocytes were collected and then cultured in TCM-199 + 10% FBS for 4 h. The matured oocytes were activated with a 20 μsec electric pulse at 1.2 kV/mm. The activated oocytes were incubated in 2 mM of 6-dimethylaminopurine (6-DMAP) for 4 h and were then divided randomly among the treatment groups. In experiment 1, we compared the effects of three culture systems (TCM-199, CR1-aa and Tyrodes) on the in vitro development of parthenogenetically activated cat oocytes. In experiment 2, we investigated the effect of addition of Iloprost (a stable prostaglandin I2 analogue) to Tyrodes medium on in vitro development of parthenogenetically activated oocytes. As a control, we recovered in vivo produced blastocysts and determined their average cell number. In experiment 1, the cleavage frequency of the oocytes cultured in TCM199, CR1-aa and Tyrodes media were similar (74, 72 and 83%, respectively). However, the incidence of in vitro development to the blastocyst stage was significantly higher in Tyrodes medium (20.4%) than in TCM-199 (2.4%) or CR1-aa (11.1%). Likewise, the average cell number of in vitro activated blastocysts was higher in Tyrodes than in CR1-aa or TCM-199 (106.5 ± 45.2 vs. 68.3 ± 25.4 and 35.0 ± 7.7, respectively; p<0.05). In experiment 2, the percentage of parthenogenetically activated oocytes that underwent in vitro blastocyst development was significantly improved by addition of Iloprost to the culture medium (33.6 vs. 19.1%; p<0.05). The average cell number of in vivo blastocysts (909.0 ± 226.4) was significantly higher than those of in vitro blastocysts cultured in Tyrodes medium supplemented with or without Iloprost (103.2 ± 31.3 and 112.2 ± 39.3, respectively; p<0.05). This result indicated that the current culture method for cat pathenogenetically activated oocytes requires further improvement.

• 日本繁殖生物学会の論文
• 2007-10-01

著者

• Yin Xi-jun

  Department Of Animal Science & Technology Sunchon National University

• JEON Jin-Tae

  Division of Applied Life Science (BK21 program), Graduate School of Gyeongsang National University

• KONG Il-Keun

  Division of Applied Life Science (BK21 program), Graduate School of Gyeongsang National University

• Kong Il-keun

  Division Of Applied Life Science (bk21 Program) Graduate School Of Gyeongsang National University

• Jeon Jin-tae

  Division Of Applied Life Science (bk21 Program) Graduate School Of Gyeongsang National University

• LEE Hyo-Sang

  Department of Animal Science and Technology, Sunchon National University

• Lee Hyo-sang

  Department Of Animal Science And Technology Sunchon National University

関連論文

• Maternal Gene Transcription in Mouse Oocytes: Genes Implicated in Oocyte Maturation and Fertilization
• Cloning and Characterization of Cat POU5F1 and NANOG for Identification of Embryonic Stem-like Cells
• RFP-トランスジェニッククローンネコの臍帯血由来間葉系細胞からの神経様細胞への分化(実験動物学)
• In Vitro Maturation of Oocytes Derived from the Brown Bear (Ursus Arctos)
• Effect of Culture Medium and Prostaglandin I_2 (PGI_2) Analogue on In Vitro Development of Parthenogenetically Activated Cat Oocytes
• Maternal Gene Transcription in Mouse Oocytes : Genes Implicated in Oocyte Maturation and Fertilization

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