Fine Surface Structure of Bovine Acrosome-Intact and Reacted Spermatozoa Observed by Atomic Force Microscopy
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概要
- 論文の詳細を見る
The atomic force microscope (AFM) provides nanometer resolution, topographic data of the natural surface structure of materials. We studied the topology of the surface structure of bovine sperm heads during the acrosome reaction by AFM. In addition, we numerically analyzed the areas of the median sagittal plane of the sperm heads. Bovine frozen-thawed spermatozoa were washed, capacitated by heparin, and incubated with lysophosphatidylcholine (LPC) to induce the acrosome reaction, smeared on a cover glass, air-dried, and observed with AFM using the dynamic force (tapping) mode. AFM analysis of spermatozoa showed the clear surface structure of acrosomes, equatorial segments, postacrosomal regions and necks. Although AFM images of spermatozoa capacitated by heparin had complete acrosomes, most spermatozoa treated with LPC had no acrosomal caps as shown by AFM. These observations coincided with those obtained by light microscopy after staining with naphthol yellow S and erythrosin B. Furthermore, numerical analysis of AFM images indicated that areas of the median sagittal plane of the anterior portions of acrosome-reacted sperm heads (2679 ± 616 pixels) were approximately 40% less than those of intact heads (4535 ± 174 pixels, P<0.05). These results indicate that AFM can usefully observe and numerically analyze the fine surface structures of bovine spermatozoa.
- 日本繁殖生物学会の論文
著者
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Nagata Yuki
Department Of Bioengineering Faculty Of Engineering Kagoshima University
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Kato Nobuhiro
Department Of Cellular And Molecular Biology Hiroshima University School Of Medicine
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Hosoi Yoshihiko
Institute Of Advanced Technology Kinki University
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IRITANI Akira
Institute of Advanced Technology, Kinki University
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SAEKI Kazuhiro
Institute of Advanced Technology, Kinki University
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MATSUMOTO Kazuya
Institute of Advanced Technology, Kinki University
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Sumitomo Norio
Department Of Genetic Engineering Kinki University
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Matsumoto Kazuya
Institute For Chemical Research Kyoto University
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KATO Nobuhiro
Department of Intelligent Systems, Kinki University
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