Purification and Characterization of a 24kDa Protein from Tartary Buckwheat Seeds

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概要

• 論文の詳細を見る

• A 24 kDa protein was isolated from tartary buckwheat seeds by using chromatography of Superdex 75 gel filtration and Resource Q ion-exchange column. SDS-PAGE and Sephacryl S-200 gel filtration were used to provide information about the molecular mass of the protein purified from tartary buckwheat. The protein was composed of 215 amino acid residues and showed strong IgE binding activity in an ELISA test to the sera colleted from two patients allergic to buckwheat. These results suggested that the purified 24 kDa protein from tartary buckwheat seeds was an important functional protein and was relatively specific for buckwheat-allergic patients. It should be a very useful tool in the diagnosis of buckwheat allergy in the future.

• 2004-07-23

著者

• Zhang Zheng

  Institute Of Biotechnology Shanxi University

• WANG Zhuanhua

  Institute of Biotechnology, Shanxi University

• WIESLANDER Gunilla

  Department of Medical Sciences/Occupational and Environment Medicine, Uppsala University, University

• NORBACK Dan

  Department of Medical Sciences/Occupational and Environment Medicine, Uppsala University, University

• WANG Zhuanhua

  College of Life Science and Biotechnology, Shanxi University

• ZHANG Zheng

  College of Life Science and Biotechnology, Shanxi University

• ZHAO Zhuohui

  College of Life Science and Biotechnology, Shanxi University

• KREFT Ivan

  Biotechnical Faculty, University of Ljubljana

• Kreft Ivan

  Biotechnical Faculty University Of Ljubljana

• Norback Dan

  Department Of Medical Sciences/occupational And Environment Medicine Uppsala University University H

• Zhao Zhuohui

  College Of Life Science And Biotechnology Shanxi University

• Wang Zhuanhua

  Institute Of Biotechnology Shanxi University

• Wieslander Gunilla

  Department Of Medical Sciences/occupational And Environment Medicine Uppsala University University H

• Kreft Ivan

  Biotechnical Fac. Univ. Of Ljubljana

• Norback Dan

  Department Of Medical Science Occupational And Environmental Medicine Uppsala University University

• Wieslander Gunilla

  Department Of Medical Science Occupational And Environmental Medicine Uppsala University University

関連論文

• A single nucleotide polymorphism of MHC region is associated with subphenotypes of Psoriasis in Chinese population
• Cloning, Expression, and Identification of Immunological Activity of an Allergenic Protein in Tartary Buckwheat
• Purification and Characterization of a 24kDa Protein from Tartary Buckwheat Seeds
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• Selenium and Its Species Distribution in Above-ground Plant Parts of Selenium Enriched Buckwheat (Fagopyrum esculentum Moench)
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• Selenium Species Determination in Selenium-Enriched Pumpkin (Cucurbita pepo L.) Seeds by HPLC-UV-HG-AFS

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