Cloning, Expression in Yeast, and Functional Characterization of CYP76A4, a Novel Cytochrome P450 of Petunia That Catalyzes (ω-1)-Hydroxylation of Lauric Acid
スポンサーリンク
概要
- 論文の詳細を見る
A cDNA clone of a novel cytochrome P450, CYP76A4, was isolated from Petunia hybrida. The cDNA clone contained an open reading frame (ORF) encoding a predicted 510 amino acid polypeptide. The CYP76A4 cDNA was expressed in yeast Saccharomyces cerevisiae AH22. Recombinant yeast microsomes containing the CYP76A4 hemoprotein were found to catalyze (ω-1)-hydroxylation of lauric acid.
- 社団法人 日本農芸化学会の論文
- 2005-02-23
著者
-
Oono Kiyoharu
Functional Analysis Of Environmental Genes Research Center For Environmental Genomics Kobe Universit
-
OHKAWA HIDEO
Department of Biological and Environmental Science, Faculty of Agriculture, Kobe University
-
TAMAKI Katsutomo
Laboratory of Biotechnology, Hyogo Prefectural Research Institute for Agriculture
-
IMAISHI Hiromasa
Functional Analysis of Environmental Genes, Research Center for Environmental Genomics, Kobe Univers
-
SUGIMOTO Mikihiro
Laboratory of Biotechnology, Hyogo Prefectural Research Institute for Agriculture
-
Ohkawa Hideo
Department Of Applied Biological Science Faculty Of Life Science Fukuyama University
-
Ohkawa Hideo
Deparment Of Biological And Environmental Science Faculty Of Agriculture Kobe University
-
Imaishi Hiromasa
Functional Analysis Of Environmental Genes Research Center For Environmental Genomics Kobe Universit
-
Tamaki Katsutomo
Laboratory Of Biotechnology Hyogo Prefectural Research Institute For Agriculture
-
Imaishi Hiromasa
Functional Analysis Of Environmental Genes Research Center For Environmental Genomics Kobe Universit
-
Sugimoto Mikihiro
Laboratory Of Biotechnology Hyogo Prefectural Research Institute For Agriculture
関連論文
- Immobilization of P450 Monooxygenase and Chloroplast for Use in Light-Driven Bioreactors
- Purified Fusion Enzyme between Rat Cytochrome P4501A1 and Yeast NADPH-Cytochrome P450 Oxidoreductase
- Regeneration of NADPH by Cactus Chloroplasts : Coupling Reaction with P450 Monooxygenase
- Cloning, Expression in Yeast, and Functional Characterization of CYP76A4, a Novel Cytochrome P450 of Petunia That Catalyzes (ω-1)-Hydroxylation of Lauric Acid
- Molecular Mechanisms of Herbicide Resistance with Special Emphasis on Cytochrome P450 Monooxygenases
- Molecular Cloning and Expression in Saccharomyces cerevisiae of Tobacco NADPH-Cytochrome P450 Oxidoreductase cDNA
- Inhibitory Effects of 1, 4-Naphthoquinone Derivatives on Rat Cytochrome P4501A1-Dependent Monooxygenase Activity in Recombinant Yeast Microsomes
- Effectiveness of Polyclonal and Monoclonal Antibodies Prepared for an Immunoassay of the Etofenprox Insecticide
- ELECTRON INTERACTION BETWEEN MICROSOMAL CYTOCHROME P450 MONOOXYGENASE AND PHOTOSYNTHETIC ELECTRON TRANSFER SYSTEM IN TOBACCO CHLOROPLASTS
- TRANSPORT OF RAT CYP1A1 AND RAT CYP1A1/YEAST NADPH-P450 OXIDOREDUCTASE FUSED ENZYME INTO TOBACCO CHLOROPLASTS AND THEIR INTERACTION WITH PHOTOSYSTEM I
- Effects of Methanol and Temperature on Enzyme Immunoassay with Monoclonal Antibodies Specific to the Insecticide Etofenprox
- Encoding of a Cytochrome P450-Dependent Lauric Acid Monooxygenase by CYP703A1 Specifically Expressed in the Floral Buds of Petunia hybrida
- CYP78A1 Preferentially Expressed in Developing Inflorescences of Zea mays Encoded a Cytochrome P450-Dependent Lauric Acid 12-Monooxygenase
- Expression of Human Cytochromes P450 1A1 and P450 1A2 as Fused Enzymes with Yeast NADPH-cytochrome P450 Oxidoreductase in Transgenic Tobacco Plants
- Expression of Arabidopsis thaliana cytochrome P450 monooxygenase, CYP71A12, in yeast catalyzes the metabolism of herbicide pyrazoxyfen