The Establishment of a HeLa Cell Demonstrating Rapid Mitogen-Activated Protein Kinase Phosphorylation in Response to 1α,25-Dihydroxyvitamin D_3 by Stable Transfection of a Chick Skeletal Muscle cDNA Library

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概要

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• 1α,25-dihydroxyvitamin D3 [1,25-(OH)2D3] phosphorylates the extracellular signal-regulated kinase (ERK), a member of the mitogen-activated protein kinase (MAPK) family, within 30 sec in primary cultured chick skeletal muscle cells. MAPK of HeLa cell lines, which had been stably transfected with a cDNA library derived from mRNA of chick skeletal muscle cells, was also rapidly phosphorylated by 1,25-(OH)2D3. These cell lines have the potential to be a good tool for further investigation of rapid non-genomic mechanism activated by 1,25-(OH)2D3.

• 社団法人 日本農芸化学会の論文
• 2006-01-23

著者

• Yamauchi Jun

  Division Of Applied Food Research National Institute Of Health And Nutrition

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• The Establishment of a HeLa Cell Demonstrating Rapid Mitogen-Activated Protein Kinase Phosphorylation in Response to 1α,25-Dihydroxyvitamin D_3 by Stable Transfection of a Chick Skeletal Muscle cDNA Library

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