Highly Induced DNA Recombination Mediated by Membrane Permeabilized Recombinant Cre Protein in Mouse Primary Cells

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概要

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• To establish efficient induction of Cre mediated DNA recombination in primary cells, mouse embryonic fibroblast, keratinocyte, and primary preosteoblast, we tested various recombinant Cres by fusing of protein transduction domain in human immunodeficiency virus (HIV) transactivator of transcription (TAT-PTD) to the N- and/or C-terminus. HTC, modified Cre with PTD at the N-terminus, achieved the highest activity of DNA recombination for those primary cells.

著者

• Hidema Shizu

  Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University

• Maruyama Takamitsu

  Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University

• Kato Shigeki

  Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University

• NISHIMORI Katsuhiko

  Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University

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